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A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas
Wang, Weilin1,3; Zhang, Tao1; Wang, Lingling2; Xu, Jiachao1,3; Li, Meijia1,3; Zhang, Anguo2; Qiu, Limei1; Song, Linsheng2
2016-12-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号65页码:182-190
文章类型Article
摘要Toll like receptors (TLRs) are evolutionarily prevalent recognition molecules in the Animalia and Plantae kingdom, which play vital roles in immune defense and homeostasis maintenance. Recently, the expansion of TLRs has been reported in invertebrate genomes, but the characters and immune functions of these expanded TLRs were still not well known. In the present study, a new member of TLR family with five LRR domains was identified in Crassostrea gigas (designated CgTLR6). It shared homology with TLRs from other organisms with the closest phylogenic relationship with molluscan TLRs. The recombinant protein of CgTLR6 (rCgTLR6) displayed direct bind activity to gram-negative bacteria Vibrio anguillarum and Vibrio splendidus, gram-positive bacteria Staphylococci aureus and Micrococcus luteus, and fungi Pichia pastoris, but not to fungi Yarrowia lipolytica. It also exhibited affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN), while no affinity to mannan (MAN). The mRNA of CgTLR6 was mainly detected in hemocytes and hepatopancreas, and was significantly induced (p < 0.01) in hemocytes after the oyster was stimulated with LPS, PGN or bacteria V. splendidus. Immunofluorescence analysis indicated that CgTLR6 was mainly located at the membrane of hemocytes. The blockage of CgTLR6 by anti-rCgTLR6 antibody did not significantly inhibit the phagocytic rates of hemocytes toward recognized gram negative bacteria V. anguillarum and V. splendidus, and unrecognized fungi Y. lipolytica. These results collectively implied that CgTLR6 was a novel non-phagocytic receptor of C. gigas to mediate humoral immune response by recognizing pathogen-associated molecular patterns on the invaders. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Cgtlr6 Recognition Spectrum Non-phagocytic Receptor Immune Response Crassostrea Gigas
DOI10.1016/j.dci.2016.07.010
收录类别SCI
语种英语
WOS记录号WOS:000384388400020
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/135888
专题实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Weilin,Zhang, Tao,Wang, Lingling,et al. A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:182-190.
APA Wang, Weilin.,Zhang, Tao.,Wang, Lingling.,Xu, Jiachao.,Li, Meijia.,...&Song, Linsheng.(2016).A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,182-190.
MLA Wang, Weilin,et al."A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):182-190.
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