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A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas
Wang, Weilin1,3; Zhang, Tao1; Wang, Lingling2; Xu, Jiachao1,3; Li, Meijia1,3; Zhang, Anguo2; Qiu, Limei1; Song, Linsheng2
2016-12-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume65Pages:182-190
SubtypeArticle
AbstractToll like receptors (TLRs) are evolutionarily prevalent recognition molecules in the Animalia and Plantae kingdom, which play vital roles in immune defense and homeostasis maintenance. Recently, the expansion of TLRs has been reported in invertebrate genomes, but the characters and immune functions of these expanded TLRs were still not well known. In the present study, a new member of TLR family with five LRR domains was identified in Crassostrea gigas (designated CgTLR6). It shared homology with TLRs from other organisms with the closest phylogenic relationship with molluscan TLRs. The recombinant protein of CgTLR6 (rCgTLR6) displayed direct bind activity to gram-negative bacteria Vibrio anguillarum and Vibrio splendidus, gram-positive bacteria Staphylococci aureus and Micrococcus luteus, and fungi Pichia pastoris, but not to fungi Yarrowia lipolytica. It also exhibited affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN), while no affinity to mannan (MAN). The mRNA of CgTLR6 was mainly detected in hemocytes and hepatopancreas, and was significantly induced (p < 0.01) in hemocytes after the oyster was stimulated with LPS, PGN or bacteria V. splendidus. Immunofluorescence analysis indicated that CgTLR6 was mainly located at the membrane of hemocytes. The blockage of CgTLR6 by anti-rCgTLR6 antibody did not significantly inhibit the phagocytic rates of hemocytes toward recognized gram negative bacteria V. anguillarum and V. splendidus, and unrecognized fungi Y. lipolytica. These results collectively implied that CgTLR6 was a novel non-phagocytic receptor of C. gigas to mediate humoral immune response by recognizing pathogen-associated molecular patterns on the invaders. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCgtlr6 Recognition Spectrum Non-phagocytic Receptor Immune Response Crassostrea Gigas
DOI10.1016/j.dci.2016.07.010
Indexed BySCI
Language英语
WOS IDWOS:000384388400020
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135888
Collection实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Weilin,Zhang, Tao,Wang, Lingling,et al. A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:182-190.
APA Wang, Weilin.,Zhang, Tao.,Wang, Lingling.,Xu, Jiachao.,Li, Meijia.,...&Song, Linsheng.(2016).A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,182-190.
MLA Wang, Weilin,et al."A new non-phagocytic TLR6 with broad recognition ligands from Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):182-190.
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