IOCAS-IR  > 实验海洋生物学重点实验室
Transcriptional activation and translocation of ancient NOS during immune response
Jiang, Qiufen1,2; Liu, Zhaoqun1,2; Zhou, Zhi1; Wang, Lingling1,3; Wang, Leilei1,2; Yue, Feng1,2; Wang, Jingjing1,2; Wang, Hao1; Song, Linsheng3
2016-10-01
发表期刊FASEB JOURNAL
卷号30期号:10页码:3527-3540
文章类型Article
摘要NOS is the key component of the NO system, which plays an indispensable role in many physiologic and immunologic processes; however, the process that underlies the activation of ancient NOSs and their functions remains unclear. Expression of Crassostrea gigas NOS (CgNOS) mRNA in hemocytes was examined after stimulating oysters with LPS and TNF-alpha Expression level of CgNOS mRNA was increased significantly, by 2.61-fold (P < 0.05), at 24 h poststimulation. A positive CgNOS signal was detected via immunoprecipitation, and only one protein was detected in oyster hemocytes. Shifting and supershifting bands were observed in EMSAs between the CgNOS promoter and the transcription factors CgNF-kappa B1 and Cg-signal transducer and activator of transcription (STAT). CgNF-kappa B1 was detected in the nucleus only at 12 h, whereas CgSTAT was observed in the cytoplasm and nucleus at 12 and 24 h. Expression levels of tyrosine-protein kinase receptor Tie-1, phosphatidylinositide phosphatase SAC2, phosphatidylinositol-4-phosphate 5-kinase type-1, diacylglycerol kinase , LPS-induced TNF-alpha factor-like protein, cAMP-dependent transcription factor-2, NF-kappa B1, and STAT6 were significantly elevated in a transcriptome analysis after 12 h of LPS and TNF-alpha stimulation. An immunoreactive CgNOS signal was observed in both the cell membrane and cytoplasm at 12 h, whereas it was mainly localized to the cytoplasm at 24 h post-LPS and -TNF-alpha stimulation. These findings revealed that CgNOS could be transcriptionally activated by CgNF-kappa B1 and CgSTAT via the PI3K-Akt pathway, similar to what occurs for iNOS, but CgNOS translocated to the cytoplasm, similar to neuronal NOS, to modulate downstream signals during an immune defense. These results collectively provide crucial knowledge about the evolution of NOS structure and function.
关键词Nitric Oxide Synthase Nf-kappa b Stat
DOI10.1096/fj.201500193RR
收录类别SCI
语种英语
WOS记录号WOS:000384329800022
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/135887
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Qiufen,Liu, Zhaoqun,Zhou, Zhi,et al. Transcriptional activation and translocation of ancient NOS during immune response[J]. FASEB JOURNAL,2016,30(10):3527-3540.
APA Jiang, Qiufen.,Liu, Zhaoqun.,Zhou, Zhi.,Wang, Lingling.,Wang, Leilei.,...&Song, Linsheng.(2016).Transcriptional activation and translocation of ancient NOS during immune response.FASEB JOURNAL,30(10),3527-3540.
MLA Jiang, Qiufen,et al."Transcriptional activation and translocation of ancient NOS during immune response".FASEB JOURNAL 30.10(2016):3527-3540.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Transcriptional acti(2342KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jiang, Qiufen]的文章
[Liu, Zhaoqun]的文章
[Zhou, Zhi]的文章
百度学术
百度学术中相似的文章
[Jiang, Qiufen]的文章
[Liu, Zhaoqun]的文章
[Zhou, Zhi]的文章
必应学术
必应学术中相似的文章
[Jiang, Qiufen]的文章
[Liu, Zhaoqun]的文章
[Zhou, Zhi]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Transcriptional activation and translocation of ancient NOS during immune response.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。