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The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas
Jiang, Shuai1; Jia, Zhihao1,3; Xin, Lusheng1,3; Sun, Ying1,3; Zhang, Ran1; Wang, Weilin1,3; Wang, Lingling2; Song, Linsheng2
2016-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume55Pages:490-498
SubtypeArticle
AbstractPhagocytes have been proved to play vital roles in the innate immune response. However, the cellular characteristics of phagocytes in invertebrates, especially in molluscs, remain largely unknown. In the present study, fluorescence activated cell sorting (FRCS) was employed to sort the phagocytes from the non-phagocytic haemocytes of the Pacific oyster Crassostrea gigas. The cytochemical staining analysis revealed that phagocytes were positive staining for alpha-naphthyl acetate esterase and myeloperoxidase, while negative staining for toluidine blue and periodic acid-Schiff. The non-phagocytic haemocytes exhibited positive staining for periodic acid-Schiff, weak positive staining for toluidine blue, but negative staining for alpha-naphthyl acetate esterase and myeloperoxidase. In addition, phagocytes exhibited ultra structural cellular features similar to those of macrophages, with large cell diameter, rough cell membrane and extended pseudopodia revealed by the scanning electron microscopy, while the non-phagocytic haemocytes exhibited small cell diameter, smooth cell surface and round spherical shape. Transmission electron microscopy further demonstrated that phagocytes were abundant of cytoplasmic bodies and mitochondria, while non-phagocytic haemocytes were characterized as the comparatively large cell nucleus with contorted and condensed heterochromatin adherent to the nuclear envelope. Moreover, compared with non-phagocytic haemocytes, phagocytes exhibited significantly higher levels of intracellular cytokines, including tumor necrosis factor, interferon-like protein and interleukin-17, and significantly higher abundance of lysosome and reactive oxygen species, which were of great importance to the activation of immune response and pathogen clearance. Taken together, these findings revealed the different cytochemical and ultrastructural features between phagocytes and non-phagocytic haemocytes in C. gigas, which would provide an important clue to investigate the mechanism of phagocytosis underlying the innate immune response. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Phagocyte Cytochemical Staining Ultrastructural Analysis Cytokine Lysosome Reactive Oxygen Species
DOI10.1016/j.fsi.2016.06.024
Indexed BySCI
Language英语
WOS IDWOS:000381537000053
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135879
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jiang, Shuai,Jia, Zhihao,Xin, Lusheng,et al. The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:490-498.
APA Jiang, Shuai.,Jia, Zhihao.,Xin, Lusheng.,Sun, Ying.,Zhang, Ran.,...&Song, Linsheng.(2016).The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,55,490-498.
MLA Jiang, Shuai,et al."The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 55(2016):490-498.
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