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A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes encapsulation
Wang, Mengqiang1; Wang, Lingling2; Huang, Mengmeng1; Yi, Qilin2; Guo, Ying1; Gai, Yunchao1; Wang, Hao1; Zhang, Huan1; Song, Linsheng2
2016-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume55Pages:10-20
SubtypeArticle
AbstractGalectins are a family of beta-galactoside binding lectins that function as pattern recognition receptors (PRRs) in innate immune system of both vertebrates and invertebrates. The cDNA of Chinese mitten crab Eriocheir sinensis galectin (designated as EsGal) was cloned via rapid amplification of cDNA ends (RACE) technique based on expressed sequence tags (ESTs) analysis. The full-length cDNA of EsGal was 999 bp. Its open reading frame encoded a polypeptide of 218 amino acids containing a GLECT/Gal-bind_lectin domain and a proline/glycine rich low complexity region. The deduced amino acid sequence and domain organization of EsGal were highly similar to those of crustacean galectins. The mRNA transcripts of EsGal were found to be constitutively expressed in a wide range of tissues and mainly in hepato-pancreas, gill and haemocytes. The mRNA expression level of EsGal increased rapidly and significantly after crabs were stimulated by different microbes. The recombinant EsGal (rEsGal) could bind various pathogen-associated molecular patterns (PAMPs), including lipopolysaccharide (LPS), peptidoglycan (PGN) and glucan (GLU), and exhibited strong activity to agglutinate Escherichia coli, Vibrio anguillarum, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and Pichia pastoris, and such agglutinating activity could be inhibited by both D-galactose and alpha-lactose. The in vitro encapsulation assay revealed that rEsGal could enhance the encapsulation of haemocytes towards agarose beads. These results collectively suggested that EsGal played crucial roles in the immune recognition and elimination of pathogens and contributed to the innate immune response against various microbes in crabs. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordEriocheir Sinensis Galectin Innate Immunity Pattern Recognition Receptor Pamps Binding Activity Microbes Agglutination Haemocytes Encapsulation
DOI10.1016/j.fsi.2016.04.019
Indexed BySCI
Language英语
WOS IDWOS:000381537000002
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135872
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Mengqiang,Wang, Lingling,Huang, Mengmeng,et al. A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes encapsulation[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:10-20.
APA Wang, Mengqiang.,Wang, Lingling.,Huang, Mengmeng.,Yi, Qilin.,Guo, Ying.,...&Song, Linsheng.(2016).A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes encapsulation.FISH & SHELLFISH IMMUNOLOGY,55,10-20.
MLA Wang, Mengqiang,et al."A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes encapsulation".FISH & SHELLFISH IMMUNOLOGY 55(2016):10-20.
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