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Establishment and characterization of a skin epidermal cell line from mud loach, Misgurnus anguillicaudatus, (MASE) and its interaction with three bacterial pathogens
Xu, Xiaohui; Sivaramasamy, Elayaraja; Jin, Songjun; Li, Fuhua; Xiang, Jianhai
2016-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume55Pages:444-451
SubtypeArticle
AbstractA continuous skin epidermal cell line from mud Loach (Misgurnus anguillicaudatus) (MASE cell line) was established with its application in bacteria infection demonstrated in this study. Primary MASE cell culture was initiated at 26 degrees C in Dulbecco's modified Eagle medium/F12 medium (1:1; pH7.2) supplemented with 20% fetal bovine serum (FBS). The primary MASE cells in spindle morphology proliferated into a confluent monolayer within 2 weeks, and were continuously subcultured even in 10% FBS- DMEM/F12 after 10 passages. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 26 degrees C. The MASE cells have been subcultured steadily over Passage 90 with a population doubling time of 533 h at Passage 60. Chromosome analysis revealed that 60.5% of MASE cells at Passage 60 maintained the normal diploid chromosome number (50) with a normal karyotype of 10m+F4sm + 36t. Bacteria from the three species (Aeromonas veronii, Vibrio parahaemolyticus and Escherichia coli) were used to investigate the interactions between bacteria and cellular hosts. The three strains could be attached to the MASE cells and replicate at different levels. A. veronii could induce apoptosis in the MASE cells, with highest adherence rate among the three strains, whereas V. parahaemolyticus could cause highest cell death rate through a nonapoptotic cell death pathway, with high level of replication. The results revealed that different bacteria could interact with the MASE cells in different manners, and divergent pathways might lie in mediating cell death when cellular hosts confronted with pathogen infection. Therefore, the MASE cell line may serve as a useful tool for studying the interaction between skin bacteria and fish cells. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordMisgurnus Anguillicaudatus Skin Epidermal Cell Line Fish Skin Bacteria Aeromonas Veronii Vibrio Parahaemolyticus
DOI10.1016/j.fsi.2016.06.009
Indexed BySCI
Language英语
WOS IDWOS:000381537000047
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135869
Collection实验海洋生物学重点实验室
AffiliationChinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xu, Xiaohui,Sivaramasamy, Elayaraja,Jin, Songjun,et al. Establishment and characterization of a skin epidermal cell line from mud loach, Misgurnus anguillicaudatus, (MASE) and its interaction with three bacterial pathogens[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:444-451.
APA Xu, Xiaohui,Sivaramasamy, Elayaraja,Jin, Songjun,Li, Fuhua,&Xiang, Jianhai.(2016).Establishment and characterization of a skin epidermal cell line from mud loach, Misgurnus anguillicaudatus, (MASE) and its interaction with three bacterial pathogens.FISH & SHELLFISH IMMUNOLOGY,55,444-451.
MLA Xu, Xiaohui,et al."Establishment and characterization of a skin epidermal cell line from mud loach, Misgurnus anguillicaudatus, (MASE) and its interaction with three bacterial pathogens".FISH & SHELLFISH IMMUNOLOGY 55(2016):444-451.
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