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Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants
Yang, Guo-Qian1,2; Chen, Yun-Mei1,2; Wang, Jin-Peng3; Guo, Cen1,2; Zhao, Lei1,2; Wang, Xiao-Yan1; Guo, Ying1,2; Li, Li3; Li, De-Zhu1; Guo, Zhen-Hua1
2016-08-04
Source PublicationPLANT METHODS
Volume12
SubtypeArticle
AbstractBackground: The double digest restriction-site associated DNA sequencing technology (ddRAD-seq) is a reduced representation sequencing technology by sampling genome-wide enzyme loci developed on the basis of next-generation sequencing. ddRAD-seq has been widely applied to SNP marker development and genotyping on animals, especially on marine animals as the original ddRAD protocol is mainly built and trained based on animal data. However, wide application of ddRAD-seq technology in plant species has not been achieved so far. Here, we aim to develop an optimized ddRAD library preparation protocol be accessible to most angiosperm plant species without much startup pre-experiment and costs.
KeywordRad-seq Ddrad Middrad Genotype-by-sequencing Next-generation Sequencing
DOI10.1186/s13007-016-0139-1
Indexed BySCI
Language英语
WOS IDWOS:000382353900001
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135865
Collection实验海洋生物学重点实验室
海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Yang, Guo-Qian,Chen, Yun-Mei,Wang, Jin-Peng,et al. Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants[J]. PLANT METHODS,2016,12.
APA Yang, Guo-Qian.,Chen, Yun-Mei.,Wang, Jin-Peng.,Guo, Cen.,Zhao, Lei.,...&Guo, Zhen-Hua.(2016).Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants.PLANT METHODS,12.
MLA Yang, Guo-Qian,et al."Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants".PLANT METHODS 12(2016).
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