IOCAS-IR  > 实验海洋生物学重点实验室
Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants
Yang, Guo-Qian1,2; Chen, Yun-Mei1,2; Wang, Jin-Peng3; Guo, Cen1,2; Zhao, Lei1,2; Wang, Xiao-Yan1; Guo, Ying1,2; Li, Li3; Li, De-Zhu1; Guo, Zhen-Hua1
2016-08-04
发表期刊PLANT METHODS
卷号12
文章类型Article
摘要Background: The double digest restriction-site associated DNA sequencing technology (ddRAD-seq) is a reduced representation sequencing technology by sampling genome-wide enzyme loci developed on the basis of next-generation sequencing. ddRAD-seq has been widely applied to SNP marker development and genotyping on animals, especially on marine animals as the original ddRAD protocol is mainly built and trained based on animal data. However, wide application of ddRAD-seq technology in plant species has not been achieved so far. Here, we aim to develop an optimized ddRAD library preparation protocol be accessible to most angiosperm plant species without much startup pre-experiment and costs.
关键词Rad-seq Ddrad Middrad Genotype-by-sequencing Next-generation Sequencing
DOI10.1186/s13007-016-0139-1
收录类别SCI
语种英语
WOS记录号WOS:000382353900001
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/135865
专题实验海洋生物学重点实验室
海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Yang, Guo-Qian,Chen, Yun-Mei,Wang, Jin-Peng,et al. Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants[J]. PLANT METHODS,2016,12.
APA Yang, Guo-Qian.,Chen, Yun-Mei.,Wang, Jin-Peng.,Guo, Cen.,Zhao, Lei.,...&Guo, Zhen-Hua.(2016).Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants.PLANT METHODS,12.
MLA Yang, Guo-Qian,et al."Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants".PLANT METHODS 12(2016).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Development of a uni(3195KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Guo-Qian]的文章
[Chen, Yun-Mei]的文章
[Wang, Jin-Peng]的文章
百度学术
百度学术中相似的文章
[Yang, Guo-Qian]的文章
[Chen, Yun-Mei]的文章
[Wang, Jin-Peng]的文章
必应学术
必应学术中相似的文章
[Yang, Guo-Qian]的文章
[Chen, Yun-Mei]的文章
[Wang, Jin-Peng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。