An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster

doi:10.1098/rsob.160059

IOCAS-IR > 实验海洋生物学重点实验室

	An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster
	Chen, Hao1,3 ; Zhou, Zhi 1; Wang, Lingling 2; Wang, Hao 1; Liu, Rui 1; Zhang, Huan 1; Song, Linsheng 1,2
	2016-08-01
发表期刊	OPEN BIOLOGY
卷号	6 期号:8
文章类型	Article
摘要	Acetylcholine (ACh) is the main neurotransmitter in the cholinergic neuroendocrine system and plays an indispensable role in modulating diverse immune responses. As important transporters in choline uptake, choline transporter-like proteins (CTLs) can control ACh synthesis and release indirectly in multiple organisms. In this study, cgi-miR-2d, an invertebrate- specific miRNA in oyster Crassostrea gigas, is proved to repress the synthesis/release of ACh by targeting CgCTL1 and choline uptake in haemocytes during the early stage of pathogen infection. In short, an opposite expression pattern between CgCTL1 and cgi-miR-2d is observed during Vibrio splendidus infection, accompanied by changes in haemolymph ACh. In addition, the expression level of CgCTL1 is found to be significantly repressed after cgi-miR-2d overexpression in vivo, while both haemocyte choline and haemolymph ACh are also decreased simultaneously, similar to the finding in CgCTL1 knock-down assay. As a result, the expression of two tumour necrosis factor-like proteins and the bacteriostatic activity of oyster haemocytes are found to be altered significantly by either gain-offunction cgi-miR-2d or knock-down of CgCTL1. To our knowledge, this is the first miRNA identified in invertebrates that can target the ancient cholinergic system and augment immune response during infection.
关键词	Mirna Mir-2 Family Choline Transporter-like Protein Acetylcholine Cholinergic System Immunomodulation
DOI	10.1098/rsob.160059
收录类别	SCI
语种	英语
WOS记录号	WOS:000382945100002
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/135841
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen, Hao,Zhou, Zhi,Wang, Lingling,et al. An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster[J]. OPEN BIOLOGY,2016,6(8).
APA	Chen, Hao.,Zhou, Zhi.,Wang, Lingling.,Wang, Hao.,Liu, Rui.,...&Song, Linsheng.(2016).An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster.OPEN BIOLOGY,6(8).
MLA	Chen, Hao,et al."An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster".OPEN BIOLOGY 6.8(2016).

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