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An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster
Chen, Hao1,3; Zhou, Zhi1; Wang, Lingling2; Wang, Hao1; Liu, Rui1; Zhang, Huan1; Song, Linsheng1,2
2016-08-01
Source PublicationOPEN BIOLOGY
Volume6Issue:8
SubtypeArticle
AbstractAcetylcholine (ACh) is the main neurotransmitter in the cholinergic neuroendocrine system and plays an indispensable role in modulating diverse immune responses. As important transporters in choline uptake, choline transporter-like proteins (CTLs) can control ACh synthesis and release indirectly in multiple organisms. In this study, cgi-miR-2d, an invertebrate- specific miRNA in oyster Crassostrea gigas, is proved to repress the synthesis/release of ACh by targeting CgCTL1 and choline uptake in haemocytes during the early stage of pathogen infection. In short, an opposite expression pattern between CgCTL1 and cgi-miR-2d is observed during Vibrio splendidus infection, accompanied by changes in haemolymph ACh. In addition, the expression level of CgCTL1 is found to be significantly repressed after cgi-miR-2d overexpression in vivo, while both haemocyte choline and haemolymph ACh are also decreased simultaneously, similar to the finding in CgCTL1 knock-down assay. As a result, the expression of two tumour necrosis factor-like proteins and the bacteriostatic activity of oyster haemocytes are found to be altered significantly by either gain-offunction cgi-miR-2d or knock-down of CgCTL1. To our knowledge, this is the first miRNA identified in invertebrates that can target the ancient cholinergic system and augment immune response during infection.
KeywordMirna Mir-2 Family Choline Transporter-like Protein Acetylcholine Cholinergic System Immunomodulation
DOI10.1098/rsob.160059
Indexed BySCI
Language英语
WOS IDWOS:000382945100002
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135841
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Hao,Zhou, Zhi,Wang, Lingling,et al. An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster[J]. OPEN BIOLOGY,2016,6(8).
APA Chen, Hao.,Zhou, Zhi.,Wang, Lingling.,Wang, Hao.,Liu, Rui.,...&Song, Linsheng.(2016).An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster.OPEN BIOLOGY,6(8).
MLA Chen, Hao,et al."An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster".OPEN BIOLOGY 6.8(2016).
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