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TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses
Tang, Xueying1,2,3,5; Huang, Baoyu1,3,5; Zhang, Linlin1,3,5; Li, Li1,4,5; Zhang, Guofan1,3,5
2016-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume56Pages:330-335
SubtypeArticle
AbstractAs a benthic filter feeder of estuaries, the immune system of oysters provides one of the best models for studying the genetic and molecular basis of the innate immune pathway in marine invertebrates and examining the influence of environmental factors on the immune system. Here, the molecular function of molluscan TANK-binding kinase-1 (TBK1) (which we named CgTBK1) was studied in the Pacific oyster, Crassostrea gigas. Compared with known TBK1 proteins in other model organisms, CgTBK1 contains a conserved S-TKc domain and a coiled coil domain at the N- and C-terminals but lacks an important ubiquitin domain. Quantitative real-time PCR analysis revealed that the expression level of CgTBK1 was ubiquitous in all selected tissues, with highest expression in the gills. CgTBK1 expression was significantly upregulated in response to infections with Vibrio alginolyticus, ostreid herpesvirus 1 (OsHV-1 reference strain and par), and polyinosinic:polycytidylic acid sodium salt, suggesting its broad function in immune response. Subcellular localization showed the presence of CgTBK1 in the cytoplasm of HeLa cells, suggesting its potential function as the signal transducer between the receptor and transcription factor. We further demonstrated that CgTBK1 interacted with CgSTING in HEK293T cells, providing evidence that CgTBK1 could be activated by direct binding to CgSTING. In summary, we characterized the TBK1 gene in C gigas and demonstrated its role in the innate immune response to pathogen infections. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordPacific Oyster Crassostrea Gigas Tbk1 Innate Immunity Sting
DOI10.1016/j.fsi.2016.07.011
Indexed BySCI
Language英语
WOS IDWOS:000383292200034
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135836
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China
5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:330-335.
APA Tang, Xueying,Huang, Baoyu,Zhang, Linlin,Li, Li,&Zhang, Guofan.(2016).TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses.FISH & SHELLFISH IMMUNOLOGY,56,330-335.
MLA Tang, Xueying,et al."TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses".FISH & SHELLFISH IMMUNOLOGY 56(2016):330-335.
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