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A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii
Zhao Xin-peng1; Hu Yong-hua2; Liu Yong1; Wang Jing-jing1; Wang Guang-hua1; Wang Ren-jie1; Zhang Min1
2016-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume56Pages:402-409
SubtypeArticle
AbstractHigh-mobility group box (HMGB) 1 is a chromosomal protein that plays critical roles in DNA transcription, replication and repair. In addition, HMGB1 functions as a pro-inflammatory molecule in many vertebrates and invertebrates. In teleosts, very limited studies of HMGB1 have been reported. In this study, we identified a HMGB1 homologue (SsHMGB1) from black rockfish (Sebastes schlegelii) and analyzed its structure, expression and biological function. The open reading frame of SsHMGB1 is 621 bp, with a 5'-untranslated region (UTR) of 62 by and a 3'-UTR of 645 bp. SsHMGB1 contains two typical HMG boxes and an acidic C-terminal tail. The deduced amino acid sequence of SsHMGB1 shares the highest overall identity (89.4%) with the HMGB1 of Anoplopoma fimbria. The expression of SsHMGB1 occurred in multiple tissues and was highest in the brain. Moreover, the mRNA level of SsHMGB1 in head kidney (HK) macrophages could be induced by Listonella anguillarum in a time-dependent manner. Recombinant SsHMGB1 purified from Escherichia coli (i) bound DNA fragments in a dose-dependent manner; and (ii) induced the expression of cytokines in HK macrophages, including a significant increase in TNF-alpha activity and enhanced mRNA level of TNF13B and IL-1 beta, which are known to be involved in antibacterial defense; moreover, (iii) significantly improved the macrophage bactericidal activity together with reduced pathogen dissemination and replication of bacteria in fish kidney. These results indicated that SsHMGB1 is a novel HMGB1 that possesses apparent immunoregulatory properties and is likely to be involved in fighting bacterial infection. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordSebastes Schlegelii High-mobility Group Box 1 Dna Binding Immunoregulation Antibacterial
DOI10.1016/j.fsi.2016.07.034
Indexed BySCI
Language英语
WOS IDWOS:000383292200043
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135834
Collection实验海洋生物学重点实验室
Affiliation1.Qingdao Agr Univ, Marine Sci & Engn Coll, 700 Changcheng Rd, Qingdao 266109, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Zhao Xin-peng,Hu Yong-hua,Liu Yong,et al. A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:402-409.
APA Zhao Xin-peng.,Hu Yong-hua.,Liu Yong.,Wang Jing-jing.,Wang Guang-hua.,...&Zhang Min.(2016).A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii.FISH & SHELLFISH IMMUNOLOGY,56,402-409.
MLA Zhao Xin-peng,et al."A high-mobility group box 1 that binds to DNA, enhances pro-inflammatory activity, and acts as an anti-infection molecule in black rockfish, Sebastes schlegelii".FISH & SHELLFISH IMMUNOLOGY 56(2016):402-409.
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