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Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis
Yang, Hui1,2; Li, Shihao1,3; Li, Fuhua1,3; Yu, Kuijie1; Yang, Fusheng4; Xiang, Jianhai1
2016-08-01
发表期刊MARINE DRUGS
卷号14期号:8
文章类型Article
摘要Anti-lipopolysaccharide factors (ALFs) with a LPS-binding domain (LBD) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. FcALF2 was one isoform of ALFs isolated from the Chinese shrimp Fenneropenaeus chinensis. Our previous study showed that a modified LBD domain (named LBDv) of FcALF2 exhibited a highly enhanced antimicrobial activity. In the present study, a modified FcALF2 gene (mFcALF2), in which the LBD was substituted by LBDv, was designed and synthesized. This gene was successfully expressed in yeast Pichia pastoris GS115 eukaryotic expression system, and the characteristics of the recombinant protein mFcALF2 were analyzed. mFcALF2 exhibited apparent antibacterial activities against Gram-negative bacteria, including Escherichia coli, Vibrio alginolyticus, Vibrio harveyi, and Vibrio parahaemolyticus, and Gram-positive bacteria, including Bacillus licheniformis and Staphylococcus epidermidis. In addition, mFcALF2 could reduce the propagation of white spot syndrome virus (WSSV) in vivo by pre-incubation with virus. The present study paves the way for developing antimicrobial drugs in aquaculture.
关键词Anti-lipopolysaccharide Factors Recombinant Protein Antibacterial Activity Antiviral Activity
DOI10.3390/md14080152
收录类别SCI
语种英语
WOS记录号WOS:000383284700004
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/135830
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Hangzhou Xiaoshan Donghai Aquaculture Co Ltd, Hangzhou 311200, Zhejiang, Peoples R China
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GB/T 7714
Yang, Hui,Li, Shihao,Li, Fuhua,et al. Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis[J]. MARINE DRUGS,2016,14(8).
APA Yang, Hui,Li, Shihao,Li, Fuhua,Yu, Kuijie,Yang, Fusheng,&Xiang, Jianhai.(2016).Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis.MARINE DRUGS,14(8).
MLA Yang, Hui,et al."Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis".MARINE DRUGS 14.8(2016).
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