IOCAS-IR  > 实验海洋生物学重点实验室
Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis
Yang, Hui1,2; Li, Shihao1,3; Li, Fuhua1,3; Yu, Kuijie1; Yang, Fusheng4; Xiang, Jianhai1
2016-08-01
Source PublicationMARINE DRUGS
Volume14Issue:8
SubtypeArticle
AbstractAnti-lipopolysaccharide factors (ALFs) with a LPS-binding domain (LBD) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. FcALF2 was one isoform of ALFs isolated from the Chinese shrimp Fenneropenaeus chinensis. Our previous study showed that a modified LBD domain (named LBDv) of FcALF2 exhibited a highly enhanced antimicrobial activity. In the present study, a modified FcALF2 gene (mFcALF2), in which the LBD was substituted by LBDv, was designed and synthesized. This gene was successfully expressed in yeast Pichia pastoris GS115 eukaryotic expression system, and the characteristics of the recombinant protein mFcALF2 were analyzed. mFcALF2 exhibited apparent antibacterial activities against Gram-negative bacteria, including Escherichia coli, Vibrio alginolyticus, Vibrio harveyi, and Vibrio parahaemolyticus, and Gram-positive bacteria, including Bacillus licheniformis and Staphylococcus epidermidis. In addition, mFcALF2 could reduce the propagation of white spot syndrome virus (WSSV) in vivo by pre-incubation with virus. The present study paves the way for developing antimicrobial drugs in aquaculture.
KeywordAnti-lipopolysaccharide Factors Recombinant Protein Antibacterial Activity Antiviral Activity
DOI10.3390/md14080152
Indexed BySCI
Language英语
WOS IDWOS:000383284700004
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135830
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Hangzhou Xiaoshan Donghai Aquaculture Co Ltd, Hangzhou 311200, Zhejiang, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Hui,Li, Shihao,Li, Fuhua,et al. Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis[J]. MARINE DRUGS,2016,14(8).
APA Yang, Hui,Li, Shihao,Li, Fuhua,Yu, Kuijie,Yang, Fusheng,&Xiang, Jianhai.(2016).Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis.MARINE DRUGS,14(8).
MLA Yang, Hui,et al."Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis".MARINE DRUGS 14.8(2016).
Files in This Item:
File Name/Size DocType Version Access License
Recombinant Expressi(10169KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Hui]'s Articles
[Li, Shihao]'s Articles
[Li, Fuhua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Hui]'s Articles
[Li, Shihao]'s Articles
[Li, Fuhua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Hui]'s Articles
[Li, Shihao]'s Articles
[Li, Fuhua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.