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Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines
Wang, Jian-Hua1,3; Yang, Cui-Yun2; Fang, Sheng-Tao2; Lu, Jian1; Quan, Chun-Shan4
2016-09-01
Source PublicationWORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume32Issue:9
SubtypeArticle
AbstractBiofilm formation can make significant effects on bacteria habits and biological functions. In this study, diketopiperazines (DKPs) produced by strain of Bacillus amyloliquefaciens Q-426 was found to inhibit biofilm formed in the gas-liquid interface. Four kinds of DKPs were extracted from B. amyloliquefaciens Q-426, and we found that 0.04 mg ml(-1) DKPs could obviously inhibit the biofilm formation of the strain. DKPs produced by B. amyloliquefaciens Q-426 made a reduction on extracellular polymeric substance (EPS) components, polysaccharides, proteins, DNAs, etc. Real-time PCR was performed to determine that whether DKPs could make an obvious effect on the expression level for genes related to biofilm formation in the strain. The relative expression level of genes tasA, epsH, epsG and remB which related to proteins, extracellular matrix, and polysaccharides, were downregulated with 0.04 mg ml(-1) DKPs, while the expression level of nuclease gene nuc was significantly upregulated. The quantitative results of the mRNA expression level for these genes concerted with the quantitative results on EPS levels. All of the experimental results ultimately indicated that DKPs could inhibit the biofilm formation of the strain B. amyloliquefaciens Q-426.
KeywordBiofilm Diketopiperazines (Dkps) Extracellular Polymeric Substance (Eps) Real-time Pcr Bacillus Amyloliquefaciens Q-426
DOI10.1007/s11274-016-2106-4
Indexed BySCI
Language英语
WOS IDWOS:000382148200004
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135828
Collection海洋环境腐蚀与与生物污损重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China
4.Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Jian-Hua,Yang, Cui-Yun,Fang, Sheng-Tao,et al. Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2016,32(9).
APA Wang, Jian-Hua,Yang, Cui-Yun,Fang, Sheng-Tao,Lu, Jian,&Quan, Chun-Shan.(2016).Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,32(9).
MLA Wang, Jian-Hua,et al."Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 32.9(2016).
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