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Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study
Chen, Muyan1; Li, Xingke1; Zhu, Aijun1; Storey, Kenneth B.2; Sun, Lina3; Gao, Tianxiang4; Wang, Tianming5
2016-09-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Volume19Pages:78-89
SubtypeArticle
AbstractMarine invertebrate aestivation is a unique strategy for summer survival in response to hot marine conditions. The sea cucumber, Apostichopus japonicus, is an excellent model marine invertebrate for studies of environmentally-induced aestivation. In the present study, we used a tandem mass tag (TMT)-coupled LC-MS/MS approach to identify and quantify the global proteome expression profile over the aestivation-arousal cycle of A. japonicus. A total of 3920 proteins were identified from the intestine of sea cucumber. Among them, 630 proteins showed significant differential expression when comparing three conditions of sea cucumbers: non aestivating (active), deep-aestivation (at least 15 days of continuous aestivation), and arousal after aestivation (renewed moving and feeding). Sea cucumbers in deep aestivation showed substantial differentially expressed proteins (143 up-regulated and 267 down-regulated proteins compared with non-aestivating controls). These differentially expressed proteins suggested that protein and phospholipid probably are major fuel sources during hypometabolism and a general attenuation of carbohydrate metabolism was observed during deep aestivation. Differentially expressed proteins also provided the first global picture of a shift in protein synthesis, protein folding, DNA binding, apoptosis, cellular transport and signaling, and cytoskeletal proteins during deep aestivation in sea cucumbers. A comparison of arousal from aestivation with deep aestivation, revealed a general reversal of the changes that occurred in aestivation for most proteins. Western blot detection further validated the significant up-regulation of HSP70 and down-regulation of methyltransferase-like protein 7A-like in deep-aestivation. Our results suggest that there is substantial post-transcriptional regulation of proteins during the aestivation arousal cycle in sea cucumbers. (C) 2016 Elsevier Inc. All rights reserved.
KeywordAestivation Sea Cucumber Proteome Tandem Mass Tag Post-transcriptional Regulation
DOI10.1016/j.cbd.2016.06.005
Indexed BySCI
Language英语
WOS IDWOS:000382416300009
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/135755
Collection实验海洋生物学重点实验室
Affiliation1.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China
2.Carleton Univ, Inst Biochem, 1125 Colonel Dr, Ottawa, ON K1S 5B6, Canada
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
4.Zhejiang Ocean Univ, Fishery Coll, Zhoushan, Peoples R China
5.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Zhoushan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Muyan,Li, Xingke,Zhu, Aijun,et al. Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2016,19:78-89.
APA Chen, Muyan.,Li, Xingke.,Zhu, Aijun.,Storey, Kenneth B..,Sun, Lina.,...&Wang, Tianming.(2016).Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,19,78-89.
MLA Chen, Muyan,et al."Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 19(2016):78-89.
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