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Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity
Sun, Yuan-Yuan1,2,3; Chi, Heng1,2; Sun, Li1,2
2016-08-23
发表期刊FRONTIERS IN MICROBIOLOGY
卷号7
文章类型Article
摘要Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity.
关键词Pseudomonas Fluorescens Filamentous Hemagglutinin Motility Adhesion Virulence Vaccine
DOI10.3389/fmicb.2010.01320
收录类别SCI
语种英语
WOS记录号WOS:000381790000002
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/135725
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
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Sun, Yuan-Yuan,Chi, Heng,Sun, Li. Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA Sun, Yuan-Yuan,Chi, Heng,&Sun, Li.(2016).Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity.FRONTIERS IN MICROBIOLOGY,7.
MLA Sun, Yuan-Yuan,et al."Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity".FRONTIERS IN MICROBIOLOGY 7(2016).
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