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The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation
Hu, Yong-hua; Sun, Li
2016-05-17
Source PublicationJOURNAL OF PROTEOMICS
Volume140Pages:100-110
SubtypeArticle
AbstractFerric uptake regulator (Fur) is an important transcriptional regulator of Gram-negative bacteria. Edwardsiella tarda is a severe fish bacterial pathogen with a broad host range that includes humans. In this study, we examined the regulatory function of Fur in E. tarda via a proteomic approach. Compared to the wild type TX01, the fur mutant TX01 Delta fur exhibited (i) retarded growth, (ii) enhanced siderophore production, (iii) increased acid tolerance, which is in contrast to observations in other bacterial species, (iv) decreased survival against oxidative stress and host serum, (v) impaired ability to inhibit host immune response, (vi) attenuated tissue infectivity and overall virulence. The deficiency of TX01 Delta fur was rescued by introduction of an exogenous fur gene. iTRAQ-based comparative proteomic analysis of TX01 Delta fur and TX01 identified 89 differentially expressed proteins that cover a wide range of functional categories including those affected by fur mutation. In addition, 16 proteins were identified for the first time to be regulated by Fur in Gram-negative bacteria. These results provide the first protein based interpretation of the global impact of Fur on the physiology and infectivity of E. tarda.
KeywordEdwardsiella Tarda Fur Virulence Stress Proteomics
DOI10.1016/j.jprot.2016.04.005
Indexed BySCI
Language英语
WOS IDWOS:000376697200009
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131086
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Hu, Yong-hua,Sun, Li. The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation[J]. JOURNAL OF PROTEOMICS,2016,140:100-110.
APA Hu, Yong-hua,&Sun, Li.(2016).The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation.JOURNAL OF PROTEOMICS,140,100-110.
MLA Hu, Yong-hua,et al."The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation".JOURNAL OF PROTEOMICS 140(2016):100-110.
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