| RestrictionDigest: A powerful Perl module for simulating genomic restriction digests | |
Wang, Jinpeng1,2,3,5 ; Li, Li1,4,5; Qi, Haigang1,3,5; Dua, Xuedi1,3,5; Zhang, Guofan1,3,5
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| 2016-05-01 | |
| 发表期刊 | ELECTRONIC JOURNAL OF BIOTECHNOLOGY
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| 卷号 | 21页码:36-42 |
| 文章类型 | Article |
| 摘要 | Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction enzymes. A key factor of this method is to determine the restriction enzyme(s). But there are few computer programs which can evaluate the usability of restriction enzymes in reduced-representation sequencing. SimRAD is an R package which can simulate the digestion of DNA sequence by restriction enzymes and return enzyme loci number as well as fragment number. But for linkage mapping analysis, enzyme loci distribution is also an important factor to evaluate the enzyme. For phylogenetic studies, comparison of the enzyme performance across multiple genomes is important. It is strongly needed to develop a simulation tool to implement these functions. |
| 关键词 | Genotyping Reduced-representation Libraries Reduced-representation Sequencing |
| DOI | 10.1016/j.ejbt.2016.02.003 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376595900005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/131085 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Peoples R China 5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Jinpeng,Li, Li,Qi, Haigang,et al. RestrictionDigest: A powerful Perl module for simulating genomic restriction digests[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2016,21:36-42. |
| APA | Wang, Jinpeng,Li, Li,Qi, Haigang,Dua, Xuedi,&Zhang, Guofan.(2016).RestrictionDigest: A powerful Perl module for simulating genomic restriction digests.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,21,36-42. |
| MLA | Wang, Jinpeng,et al."RestrictionDigest: A powerful Perl module for simulating genomic restriction digests".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 21(2016):36-42. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| RestrictionDigest_ A（510KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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