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Invertase-mediated system for simple and rapid detection of pathogen
Wan, Yi; Qi, Peng; Zeng, Yan; Sun, Yan; Zhang, Dun
2016-10-05
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号233页码:454-458
文章类型Article
摘要Portable, low-cost and quantitative analysis of pathogen at home and in the field remain a key challenge in medical diagnostics and food safety. Despite many years of research, very few such tools are available. Motivated by present clinical requirements, pocket-sized device, such as commercial glucose meter (CGM), have emerged. Taking advantage of the CGM, we develop an approach to quantify bacteria. Molecular recognition elements, such as aptamer, coupled with a signal amplification method based on invertase-promoted hydrolysis of sucrose for the sensitive and selective detection of bacteria. This paper aims to develop a portable device combined with enzyme-mediated enhancement for biological/chemical analyte detection. A linear relationship between the response and the logarithm of the Staphylococcus aureus concentration using this method was obtained for concentrations ranging from 1.2 x 10(5) to 1.2 x 10(8) cfu mL(-1), with a detection limit of 1.0 x 10(5) cfu mL(-1). This study demonstrates an approach for bacteria detection and also provides a powerful platform to monitor bacterial infection, thus rendering potential broad antibacterial applications and bacteria diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
关键词Commercial Glucose Meter Bacteria Detection Invertase Aptamer
DOI10.1016/j.snb.2016.04.098
收录类别SCI
语种英语
WOS记录号WOS:000376384100034
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/131084
专题海洋腐蚀与防护研究发展中心
作者单位Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
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Wan, Yi,Qi, Peng,Zeng, Yan,et al. Invertase-mediated system for simple and rapid detection of pathogen[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,233:454-458.
APA Wan, Yi,Qi, Peng,Zeng, Yan,Sun, Yan,&Zhang, Dun.(2016).Invertase-mediated system for simple and rapid detection of pathogen.SENSORS AND ACTUATORS B-CHEMICAL,233,454-458.
MLA Wan, Yi,et al."Invertase-mediated system for simple and rapid detection of pathogen".SENSORS AND ACTUATORS B-CHEMICAL 233(2016):454-458.
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