IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
Invertase-mediated system for simple and rapid detection of pathogen
Wan, Yi; Qi, Peng; Zeng, Yan; Sun, Yan; Zhang, Dun
AbstractPortable, low-cost and quantitative analysis of pathogen at home and in the field remain a key challenge in medical diagnostics and food safety. Despite many years of research, very few such tools are available. Motivated by present clinical requirements, pocket-sized device, such as commercial glucose meter (CGM), have emerged. Taking advantage of the CGM, we develop an approach to quantify bacteria. Molecular recognition elements, such as aptamer, coupled with a signal amplification method based on invertase-promoted hydrolysis of sucrose for the sensitive and selective detection of bacteria. This paper aims to develop a portable device combined with enzyme-mediated enhancement for biological/chemical analyte detection. A linear relationship between the response and the logarithm of the Staphylococcus aureus concentration using this method was obtained for concentrations ranging from 1.2 x 10(5) to 1.2 x 10(8) cfu mL(-1), with a detection limit of 1.0 x 10(5) cfu mL(-1). This study demonstrates an approach for bacteria detection and also provides a powerful platform to monitor bacterial infection, thus rendering potential broad antibacterial applications and bacteria diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
KeywordCommercial Glucose Meter Bacteria Detection Invertase Aptamer
Indexed BySCI
WOS IDWOS:000376384100034
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wan, Yi,Qi, Peng,Zeng, Yan,et al. Invertase-mediated system for simple and rapid detection of pathogen[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,233:454-458.
APA Wan, Yi,Qi, Peng,Zeng, Yan,Sun, Yan,&Zhang, Dun.(2016).Invertase-mediated system for simple and rapid detection of pathogen.SENSORS AND ACTUATORS B-CHEMICAL,233,454-458.
MLA Wan, Yi,et al."Invertase-mediated system for simple and rapid detection of pathogen".SENSORS AND ACTUATORS B-CHEMICAL 233(2016):454-458.
Files in This Item:
File Name/Size DocType Version Access License
Invertase-mediated s(724KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wan, Yi]'s Articles
[Qi, Peng]'s Articles
[Zeng, Yan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wan, Yi]'s Articles
[Qi, Peng]'s Articles
[Zeng, Yan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wan, Yi]'s Articles
[Qi, Peng]'s Articles
[Zeng, Yan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Invertase-mediated system for simple and rapid detection of pathogen.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.