| A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing | |
Wang, Jinpeng1,2,3,5 ; Li, Li1,4,5; Zhang, Guofan1,3,5
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| 2016-05-01 | |
| 发表期刊 | G3-GENES GENOMES GENETICS
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| 卷号 | 6期号:5页码:1417-1426 |
| 文章类型 | Article |
| 摘要 | Oysters are among the most important species in global aquaculture. Crassostrea gigas, and its subspecies C. angulata, are the major cultured species. To determine the genetic basis of growth-related traits in oysters, we constructed a second-generation linkage map from 3367 single-nucleotide polymorphisms (SNPs) based on genotyping-by-sequencing, genotyped from a C. gigas x C. angulata hybrid family. These 3367 SNPs were distributed on 1695 markers, which were assigned to 10 linkage groups. The genetic linkage map had a total length of 1084.3 cM, with an average of 0.8 cM between markers; it thus represents the densest genetic map constructed for oysters to date. Twenty-seven quantitative trait loci (QTL) for five growth-related traits were detected. These QTL could explain 4.2-7.7% (mean = 5.4%) of the phenotypic variation. In total, 50.8% of phenotypic variance for shell width, 7.7% for mass weight, and 34.1% for soft tissue weight were explained. The detected QTL were distributed among eight linkage groups, and more than half (16) were concentrated within narrow regions in their respective linkage groups. Thirty-eight annotated genes were identified within the QTL regions, two of which are key genes for carbohydrate metabolism. Other genes were found to participate in assembly and regulation of the actin cytoskeleton, signal transduction, and regulation of cell differentiation and development. The newly developed high-density genetic map, and the QTL and candidate genes identified provide a valuable genetic resource and a basis for marker-assisted selection for C. gigas and C. angulata. |
| 关键词 | Single Nucleotide Polymorphisms Reduced-representation Sequencing Distortion Of Segregation Ratios Missing Data Imputation Growth-related Traits Candidate Genes |
| DOI | 10.1534/g3.116.026971 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376294400025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/131075 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China 5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Jinpeng,Li, Li,Zhang, Guofan. A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing[J]. G3-GENES GENOMES GENETICS,2016,6(5):1417-1426. |
| APA | Wang, Jinpeng,Li, Li,&Zhang, Guofan.(2016).A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing.G3-GENES GENOMES GENETICS,6(5),1417-1426. |
| MLA | Wang, Jinpeng,et al."A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing".G3-GENES GENOMES GENETICS 6.5(2016):1417-1426. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A High-Density SNP G（979KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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