IOCAS-IR  > 海洋生物技术研发中心
A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing
Wang, Jinpeng1,2,3,5; Li, Li1,4,5; Zhang, Guofan1,3,5
2016-05-01
Source PublicationG3-GENES GENOMES GENETICS
Volume6Issue:5Pages:1417-1426
SubtypeArticle
AbstractOysters are among the most important species in global aquaculture. Crassostrea gigas, and its subspecies C. angulata, are the major cultured species. To determine the genetic basis of growth-related traits in oysters, we constructed a second-generation linkage map from 3367 single-nucleotide polymorphisms (SNPs) based on genotyping-by-sequencing, genotyped from a C. gigas x C. angulata hybrid family. These 3367 SNPs were distributed on 1695 markers, which were assigned to 10 linkage groups. The genetic linkage map had a total length of 1084.3 cM, with an average of 0.8 cM between markers; it thus represents the densest genetic map constructed for oysters to date. Twenty-seven quantitative trait loci (QTL) for five growth-related traits were detected. These QTL could explain 4.2-7.7% (mean = 5.4%) of the phenotypic variation. In total, 50.8% of phenotypic variance for shell width, 7.7% for mass weight, and 34.1% for soft tissue weight were explained. The detected QTL were distributed among eight linkage groups, and more than half (16) were concentrated within narrow regions in their respective linkage groups. Thirty-eight annotated genes were identified within the QTL regions, two of which are key genes for carbohydrate metabolism. Other genes were found to participate in assembly and regulation of the actin cytoskeleton, signal transduction, and regulation of cell differentiation and development. The newly developed high-density genetic map, and the QTL and candidate genes identified provide a valuable genetic resource and a basis for marker-assisted selection for C. gigas and C. angulata.
KeywordSingle Nucleotide Polymorphisms Reduced-representation Sequencing Distortion Of Segregation Ratios Missing Data Imputation Growth-related Traits Candidate Genes
DOI10.1534/g3.116.026971
Indexed BySCI
Language英语
WOS IDWOS:000376294400025
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131075
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China
5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Jinpeng,Li, Li,Zhang, Guofan. A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing[J]. G3-GENES GENOMES GENETICS,2016,6(5):1417-1426.
APA Wang, Jinpeng,Li, Li,&Zhang, Guofan.(2016).A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing.G3-GENES GENOMES GENETICS,6(5),1417-1426.
MLA Wang, Jinpeng,et al."A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing".G3-GENES GENOMES GENETICS 6.5(2016):1417-1426.
Files in This Item:
File Name/Size DocType Version Access License
A High-Density SNP G(979KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jinpeng]'s Articles
[Li, Li]'s Articles
[Zhang, Guofan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jinpeng]'s Articles
[Li, Li]'s Articles
[Zhang, Guofan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jinpeng]'s Articles
[Li, Li]'s Articles
[Zhang, Guofan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas x Crassostrea angulata) Using Genotyping-by-Sequencing.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.