IOCAS-IR  > 实验海洋生物学重点实验室
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Liu, Ge1,2,3; Kuang, Shan1,2; Wu, Shimei4; Jin, Weihua1,2; Sun, Chaomin1,2
2016-05-24
Source PublicationSCIENTIFIC REPORTS
Volume6
SubtypeArticle
AbstractMany polysaccharides isolated from plants have exhibited promising antitumor activities. The aim of this study is to investigate the antitumor activity of the novel polysaccharide named SPS from Sargassum integerrimum, elucidate the underlying anticancer mechanism in a human lung cancer cell line A549, and evaluate its anti-angiogenic activity both in vitro and in vivo. The results show that SPS significantly reduces A549 cells viability in a dose-and time-dependent manner via MTT method. Flow cytometry analysis indicates that SPS could induce cell apoptosis, the loss of mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS) and G2/M phase cell cycle arrest of A549 cells. Up-regulation of the expressions of P53 and Bax, down-regulation of the expression of Bcl-2, and activation of cleaved caspase-3, caspase-9 and PARP are also detected by western blotting after the treatment of SPS. In addition, SPS inhibits the proliferation, migration and cord formation of human umbilical vein endothelial cells (HUVECs) in vitro, and prevents the vascular development of zebrafish embryos in vivo. Altogether, our data prove the anticancer and anti-angiogenesis properties of SPS, and provide further insights into the potential pharmacological application of SPS as antitumor and antiangiogenic agent against lung cancer.
DOI10.1038/srep26722
Indexed BySCI
Language英语
WOS IDWOS:000376390900001
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131071
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Ge,Kuang, Shan,Wu, Shimei,et al. A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo[J]. SCIENTIFIC REPORTS,2016,6.
APA Liu, Ge,Kuang, Shan,Wu, Shimei,Jin, Weihua,&Sun, Chaomin.(2016).A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo.SCIENTIFIC REPORTS,6.
MLA Liu, Ge,et al."A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo".SCIENTIFIC REPORTS 6(2016).
Files in This Item:
File Name/Size DocType Version Access License
A novel polysacchari(1790KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Ge]'s Articles
[Kuang, Shan]'s Articles
[Wu, Shimei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Ge]'s Articles
[Kuang, Shan]'s Articles
[Wu, Shimei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Ge]'s Articles
[Kuang, Shan]'s Articles
[Wu, Shimei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.