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Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1
Lou, Sulin1; Wang, Liuying1; He, Lijuan1; Wang, Zhaokai1; Wang, Guangce2,3; Lin, Xiangzhi1
2016-06-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
卷号28期号:3页码:1657-1665
文章类型Article
摘要Crocetin is one of the major active ingredients of the rare medicinal herb saffron (Crocus sativus L.), also known as crocus. Crocetin has a wide range of effects including antitumor, anticancer, antioxidant, and antiatherosclerotic activity. Crocetin is currently only obtained via extraction from crocus stigmas, with limited yields. This study developed a new method of production of crocetin from genetically engineered Chlorella vulgaris, a unicellular, fast-growing, and heterotrophically cultured green microalga. A plant expression vector carrying the genes crtRB and ZCD1, which encode enzymes controlling critical steps in crocetin biosynthesis, was introduced into C. vulgaris using the Agrobacterium tumefaciens-mediated transformation method. After hygromycin screening, resistant C. vulgaris strains were achieved. Polymerase chain reaction amplification confirmed that the crtRB and ZCD1 were successfully integrated into the C. vulgaris genome. High-performance liquid chromatography analysis of methanol extraction showed that the transgenic C. vulgaris was able to produce crocetin, while wild-type C. vulgaris was not. These results strongly suggest that introducing crtRB and ZCD1 into C. vulgaris can successfully produce crocetin. This is a new method of crocetin production in C. vulgaris to compensate for the shortage of crocetin.
关键词Chlorella Vulgaris Crtrb Zcd1 Genetic Engineering Crocetin
DOI10.1007/s10811-015-0730-2
收录类别SCI
语种英语
WOS记录号WOS:000375315100021
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/131065
专题实验海洋生物学重点实验室
作者单位1.State Ocean Adm, Engn Res Ctr Marine Biol Resource Comprehens Util, Inst Oceanog 3, 184 Daxue Rd, Xiamen 361005, Fujian Province, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
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Lou, Sulin,Wang, Liuying,He, Lijuan,et al. Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(3):1657-1665.
APA Lou, Sulin,Wang, Liuying,He, Lijuan,Wang, Zhaokai,Wang, Guangce,&Lin, Xiangzhi.(2016).Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1.JOURNAL OF APPLIED PHYCOLOGY,28(3),1657-1665.
MLA Lou, Sulin,et al."Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1".JOURNAL OF APPLIED PHYCOLOGY 28.3(2016):1657-1665.
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