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Conserved hemopoietic transcription factor Cg-SCL delineates hematopoiesis of Pacific oyster Crassostrea gigas
Song, Xiaorui1,3; Wang, Hao1; Chen, Hao1,3; Sun, Mingzhe1; Liang, Zhongxiu1; Wang, Lingling1; Song, Linsheng2
2016-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume51Pages:180-188
SubtypeArticle
AbstractHemocytes are the effective immunocytes in bivalves, which have been reported to be derived from stem-like cells in gill epithelium of oyster. In the present work, a conserved haematopoietic transcription factor Tal-1/Scl (Stem Cell Leukemia) was identified in Pacific oyster (Cg-SCL), and it was evolutionarily close to the orthologs in deuterostomes. Cg-SCL was highly distributed in the hemocytes as well as gill and mantle. The hemocyte specific genes Integrin, EcSOD and haematopoietic transcription factors GATA3, C-Myb, c-kit, were down-regulated when Cg-SCL was interfered by dsRNA. During the larval developmental stages, the mRNA transcripts of Cg-SCL gradually increased after fertilization and peaked at early trochophore larvae stage (10 hpf, hours post fertilization), then sharply decreased in late trochophore larvae stage (15 hpf) before resuming in umbo larvae (120 hpf). Whole-mount immunofluorescence assay further revealed that the immunoreactivity of Cg-SCL appeared in blastula larvae with two approximate symmetric spots, and this expression pattern lasted in gastrula larvae. By trochophore, the immunoreactivity formed a ring around the dorsal region and then separated into two remarkable spots at the dorsal side in D-veliger larvae. After bacterial challenge, the mRNA expression levels of Cg-SCL were significantly up-regulated in the D-veliger and umbo larvae, indicating the available hematopoietic regulation in oyster larvae. These results demonstrated that Cg-SCL could be used as haematopoietic specific marker to trace potential developmental events of hematopoiesis during ontogenesis of oyster, which occurred early in blastula stage and maintained until D-veliger larvae. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordHematopoiesis Pacific Oyster Transcription Factor Tal-1/scl Larval Developmental Stages Mrna Expression
DOI10.1016/j.fsi.2016.02.023
Indexed BySCI
Language英语
WOS IDWOS:000374617700021
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131062
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song, Xiaorui,Wang, Hao,Chen, Hao,et al. Conserved hemopoietic transcription factor Cg-SCL delineates hematopoiesis of Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,51:180-188.
APA Song, Xiaorui.,Wang, Hao.,Chen, Hao.,Sun, Mingzhe.,Liang, Zhongxiu.,...&Song, Linsheng.(2016).Conserved hemopoietic transcription factor Cg-SCL delineates hematopoiesis of Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,51,180-188.
MLA Song, Xiaorui,et al."Conserved hemopoietic transcription factor Cg-SCL delineates hematopoiesis of Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 51(2016):180-188.
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