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Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix
Nie, Qing1,3; Yue, Xin1,2; Liu, Baozhong1,2
2016-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume51Pages:46-52
SubtypeArticle
AbstractAs a bHLH transcriptional repressor, Hairy-related proteins can bind to DNA sites in target gene promoters and negatively regulate gene transcription. In the present study, the full-length cDNA of Hairy was obtained from the clam Meretrix meretrix (MmeHairy), and two SNPs in the 3'-untranslated region (UTR) of this gene, SNP1066 and 1067, were identified and characterized. Multiple sequence alignment and phylogenetic analysis revealed that MmeHairy belongs to the Hairy protein subfamily. Analysis of tissue expression patterns showed that the mRNA of MmeHairy had the highest expression level in the hepatopancreas. The expression levels of MmeHairy were up-regulated in the hepatopancreas after Vibrio challenge. Genotyping and quantitative analysis showed that the mRNA levels of MmeHairy were significantly different among individual clams with different genotypes at SNP1066 and 1067 (P < 0.05), which indicated that these two SNP loci may affect the expression of MmeHairy and could be used as candidate markers for future selection in M. meretrix breeding programs. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordMeretrix Meretrix Hairy Single Nucleotide Polymorphism (Snp) Vibrio-resistance
DOI10.1016/j.fsi.2016.02.003
Indexed BySCI
Language英语
WOS IDWOS:000374617700007
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131059
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Yancheng Inst Technol, Dept Marine Sci & Technol, Coll Marine & Biol Engn, Yancheng 224051, Peoples R China
First Author Affilication中国科学院海洋研究所
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Nie, Qing,Yue, Xin,Liu, Baozhong. Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix[J]. FISH & SHELLFISH IMMUNOLOGY,2016,51:46-52.
APA Nie, Qing,Yue, Xin,&Liu, Baozhong.(2016).Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix.FISH & SHELLFISH IMMUNOLOGY,51,46-52.
MLA Nie, Qing,et al."Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix".FISH & SHELLFISH IMMUNOLOGY 51(2016):46-52.
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