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Effects of hydrostatic pressure on microtubule organization and nucleus changes in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus)
Lin, Zhengmei1; Zhu, Xiangping1; Zhang, Tingrong1; You, Feng2; Wu, Zhihao2; Cao, Yuanshui3
2016-06-01
Source PublicationTHERIOGENOLOGY
Volume85Issue:9Pages:1610-1624
SubtypeArticle
AbstractFluorescent double-labeled technique was used to investigate the effects of hydrostatic pressure on microtubule organization and nucleus in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus). The parameter of hydrostatic pressure treatment was 600 kg/cm(2) for 6 minutes at prometaphase of the first mitosis. The data showed that nucleus and microtubule changes of the diploid control were basically similar to those of the haploid one (5 minutes behind those of the diploid control). Nuclear diameter of the haploid embryo was significantly smaller than that of the diploid one (P < 0.01). The ploidy of chromosome set could be determined basing on nuclear diameter. The results of nuclear diameter measurement and the ratio of developmentally delayed embryo showed that the chromosome set was not doubled during the second cell cycle, the first cleavage proceeded normally; but that of about 80% treated embryo was doubled during the third cell cycle, the second cleavage was inhibited. Microtubules were disassembled, and nucleation capacity of centrosome was just temporarily inhibited by pressure treatment. Centrosome renucleated microtubule, and a bipolar spindle reassembled 15 minutes after treatment, leading to occurrence of the first cleavage. During the second cell cycle, about 80% treated embryo had a single centrosome and formed a unipolar spindle in both blastomeres. After prometaphase, chromosomes spread around for about 20 minutes instead of aligning on the equatorial plane, then assembled and formed one large nucleus without anaphase separation. The second cleavage was inhibited, and the chromosome set was doubled. The data indicated that the chromosome set doubling of mitogynogenetic diploid induced by hydrostatic pressure treatment, which performed at prometaphase of the first mitosis, mainly resulted from the inhibition of the second cleavage rather than the first one. This study is the first to adapt fluorescent double-labeled technique to investigate the mechanism on chromosome set doubling of mitotic gynogenesis induction. This study will offer theoretical support for mitogynogenetic diploid induction in marine fish. (C) 2016 Elsevier Inc. All rights reserved.
KeywordOlive Flounder (Paralichthys Olivaceus) Mitogynogenetic Diploid Fluorescent Double-labeled Technique Hydrostatic Pressure
DOI10.1016/j.theriogenology.2016.01.020
Indexed BySCI
Language英语
WOS IDWOS:000375736100013
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131050
Collection实验海洋生物学重点实验室
Affiliation1.Qingdao Agr Univ, Dept Anim Sci & Technol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China
Recommended Citation
GB/T 7714
Lin, Zhengmei,Zhu, Xiangping,Zhang, Tingrong,et al. Effects of hydrostatic pressure on microtubule organization and nucleus changes in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus)[J]. THERIOGENOLOGY,2016,85(9):1610-1624.
APA Lin, Zhengmei,Zhu, Xiangping,Zhang, Tingrong,You, Feng,Wu, Zhihao,&Cao, Yuanshui.(2016).Effects of hydrostatic pressure on microtubule organization and nucleus changes in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus).THERIOGENOLOGY,85(9),1610-1624.
MLA Lin, Zhengmei,et al."Effects of hydrostatic pressure on microtubule organization and nucleus changes in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus)".THERIOGENOLOGY 85.9(2016):1610-1624.
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