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Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction
Zeng, Yan1,2; Qi, Peng1; Wan, Yi1; Zhang, Dun1
2016-08-01
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
Volume231Pages:675-679
SubtypeArticle
AbstractA sensitive bacterial DNA detection method based on lysozyme signal probe and exonuclease III (Exo-III) aided cycling amplification is developed. First, the capture DNA conjugated with lysozyme is assembled on magnetic beads (MBs). In the presence of a perfectly matched target bacterial DNA, blunt 3'-terminus of the capture probe is formed, thereby activating Exo-III aided cycling amplification. Thus, Exo-III catalyzes the stepwise removal of mononucleotides from this terminus, releasing lysozyme and target DNA. The released target DNA then finds another capture probe to start a new cycle, while the released lysozyme is recovered by magnetic separation for fluorescence signal collection. Because of the separating function of MBs, unique cleavage function of Exo-III, and catalytic signal amplification of lysozyme, this system achieves sensitive bacterial DNA detection, with a detection limit of 3.5 fM. Besides this sensitivity, this strategy exhibits excellent selectivity with mismatched bacterial DNA targets and other bacterial species targets, as well as good applicability in real human serum samples. As a result, this strategy shows considerable potential for qualitative and quantitative analysis of bacteria. (C) 2016 Elsevier B.V. All rights reserved.
KeywordLysozyme Magnetic Beads Exonuclease Iii Bacterial Dna Detection
DOI10.1016/j.snb.2016.03.095
Indexed BySCI
Language英语
WOS IDWOS:000374330900078
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131043
Collection海洋环境腐蚀与与生物污损重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zeng, Yan,Qi, Peng,Wan, Yi,et al. Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,231:675-679.
APA Zeng, Yan,Qi, Peng,Wan, Yi,&Zhang, Dun.(2016).Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction.SENSORS AND ACTUATORS B-CHEMICAL,231,675-679.
MLA Zeng, Yan,et al."Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction".SENSORS AND ACTUATORS B-CHEMICAL 231(2016):675-679.
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