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Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction
Zeng, Yan1,2; Qi, Peng1; Wan, Yi1; Zhang, Dun1
2016-08-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号231页码:675-679
文章类型Article
摘要A sensitive bacterial DNA detection method based on lysozyme signal probe and exonuclease III (Exo-III) aided cycling amplification is developed. First, the capture DNA conjugated with lysozyme is assembled on magnetic beads (MBs). In the presence of a perfectly matched target bacterial DNA, blunt 3'-terminus of the capture probe is formed, thereby activating Exo-III aided cycling amplification. Thus, Exo-III catalyzes the stepwise removal of mononucleotides from this terminus, releasing lysozyme and target DNA. The released target DNA then finds another capture probe to start a new cycle, while the released lysozyme is recovered by magnetic separation for fluorescence signal collection. Because of the separating function of MBs, unique cleavage function of Exo-III, and catalytic signal amplification of lysozyme, this system achieves sensitive bacterial DNA detection, with a detection limit of 3.5 fM. Besides this sensitivity, this strategy exhibits excellent selectivity with mismatched bacterial DNA targets and other bacterial species targets, as well as good applicability in real human serum samples. As a result, this strategy shows considerable potential for qualitative and quantitative analysis of bacteria. (C) 2016 Elsevier B.V. All rights reserved.
关键词Lysozyme Magnetic Beads Exonuclease Iii Bacterial Dna Detection
DOI10.1016/j.snb.2016.03.095
收录类别SCI
语种英语
WOS记录号WOS:000374330900078
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/131043
专题海洋腐蚀与防护研究发展中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
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GB/T 7714
Zeng, Yan,Qi, Peng,Wan, Yi,et al. Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,231:675-679.
APA Zeng, Yan,Qi, Peng,Wan, Yi,&Zhang, Dun.(2016).Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction.SENSORS AND ACTUATORS B-CHEMICAL,231,675-679.
MLA Zeng, Yan,et al."Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction".SENSORS AND ACTUATORS B-CHEMICAL 231(2016):675-679.
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