Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction | |
Zeng, Yan1,2 ; Qi, Peng1; Wan, Yi1; Zhang, Dun1
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| 2016-08-01 | |
| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| 卷号 | 231页码:675-679 |
| 文章类型 | Article |
| 摘要 | A sensitive bacterial DNA detection method based on lysozyme signal probe and exonuclease III (Exo-III) aided cycling amplification is developed. First, the capture DNA conjugated with lysozyme is assembled on magnetic beads (MBs). In the presence of a perfectly matched target bacterial DNA, blunt 3'-terminus of the capture probe is formed, thereby activating Exo-III aided cycling amplification. Thus, Exo-III catalyzes the stepwise removal of mononucleotides from this terminus, releasing lysozyme and target DNA. The released target DNA then finds another capture probe to start a new cycle, while the released lysozyme is recovered by magnetic separation for fluorescence signal collection. Because of the separating function of MBs, unique cleavage function of Exo-III, and catalytic signal amplification of lysozyme, this system achieves sensitive bacterial DNA detection, with a detection limit of 3.5 fM. Besides this sensitivity, this strategy exhibits excellent selectivity with mismatched bacterial DNA targets and other bacterial species targets, as well as good applicability in real human serum samples. As a result, this strategy shows considerable potential for qualitative and quantitative analysis of bacteria. (C) 2016 Elsevier B.V. All rights reserved. |
| 关键词 | Lysozyme Magnetic Beads Exonuclease Iii Bacterial Dna Detection |
| DOI | 10.1016/j.snb.2016.03.095 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374330900078 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/131043 |
| 专题 | 海洋环境腐蚀与生物污损重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zeng, Yan,Qi, Peng,Wan, Yi,et al. Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,231:675-679. |
| APA | Zeng, Yan,Qi, Peng,Wan, Yi,&Zhang, Dun.(2016).Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction.SENSORS AND ACTUATORS B-CHEMICAL,231,675-679. |
| MLA | Zeng, Yan,et al."Sensitive quantitative detection of bacterial DNA based on lysozyme signal probe and Exo III-aided cycling amplification reaction".SENSORS AND ACTUATORS B-CHEMICAL 231(2016):675-679. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Sensitive quantitati（972KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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