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Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium
Li, Meng1,2,3; Wang, Jinfeng1,2; Song, Shuqun1,2; Li, Caiwen1,2
2016-05-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号52页码:263-277
文章类型Article
摘要Increasing evidences have established that the nitric oxide synthase (NOS) and NADPH oxidase (NOX) play important roles in host defense system by catalyzing the production of nitrogen oxide (NO) and superoxide anions (O-2(-)), respectively. While, there are limited studies to explore the roles of NOS/NOX enzymes in crustacean immunity, and no studies as yet were attempted to elucidate their functions in host immune responses to parasites. In the present study, we cloned a full-length cDNA of NOS and two partial cDNA fragments of NOX and GPx from the economic valuable crab Portunus trituberculatus. The full-length cDNA of NOS was 4002 bp in length that encoded 1203 amino acids containing motifs of the NOS protein and conserved domains. The phylogenetic analysis showed that the NOS protein sequence was clustered together with those of crustacean species in the phylogenetic tree. All of the three novel genes showed high mRNA transcripts in the immune-related tissues (e.g. hemocytes, hepatopancreas) of P. trituberculatus. Striking fluctuation in the transcripts of the critical NO/O-2(-)- generating/scavenging related genes (NOS, NOX, CuZnSOD, CAT, GPx) as well as in the enzymatic activities of NOS, NOX, SOD, CAT and GPx were observed in the hemocytes and hepatopancreas of P. trituberculatus post challenged with the parasitic dinoflagellate Hematodinium, indicating that the NO/O-2(-)- generating and the antioxidant systems played vital roles in the crustacean innate immunity against the parasitic intrusion. The results indicated a novel respect of the host-parasite interaction between the crab host and the parasitic dinoflagellate Hematodinium. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Crustacean Innate Immunity Parasites Nitric Oxide Synthase Nadph Oxidase Antioxidant Enzymes
DOI10.1016/j.fsi.2016.03.042
收录类别SCI
语种英语
WOS记录号WOS:000375165200028
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/131032
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Li, Meng,Wang, Jinfeng,Song, Shuqun,et al. Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium[J]. FISH & SHELLFISH IMMUNOLOGY,2016,52:263-277.
APA Li, Meng,Wang, Jinfeng,Song, Shuqun,&Li, Caiwen.(2016).Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium.FISH & SHELLFISH IMMUNOLOGY,52,263-277.
MLA Li, Meng,et al."Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium".FISH & SHELLFISH IMMUNOLOGY 52(2016):263-277.
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