IOCAS-IR  > 海洋生态与环境科学重点实验室
Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium
Li, Meng1,2,3; Wang, Jinfeng1,2; Song, Shuqun1,2; Li, Caiwen1,2
2016-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume52Pages:263-277
SubtypeArticle
AbstractIncreasing evidences have established that the nitric oxide synthase (NOS) and NADPH oxidase (NOX) play important roles in host defense system by catalyzing the production of nitrogen oxide (NO) and superoxide anions (O-2(-)), respectively. While, there are limited studies to explore the roles of NOS/NOX enzymes in crustacean immunity, and no studies as yet were attempted to elucidate their functions in host immune responses to parasites. In the present study, we cloned a full-length cDNA of NOS and two partial cDNA fragments of NOX and GPx from the economic valuable crab Portunus trituberculatus. The full-length cDNA of NOS was 4002 bp in length that encoded 1203 amino acids containing motifs of the NOS protein and conserved domains. The phylogenetic analysis showed that the NOS protein sequence was clustered together with those of crustacean species in the phylogenetic tree. All of the three novel genes showed high mRNA transcripts in the immune-related tissues (e.g. hemocytes, hepatopancreas) of P. trituberculatus. Striking fluctuation in the transcripts of the critical NO/O-2(-)- generating/scavenging related genes (NOS, NOX, CuZnSOD, CAT, GPx) as well as in the enzymatic activities of NOS, NOX, SOD, CAT and GPx were observed in the hemocytes and hepatopancreas of P. trituberculatus post challenged with the parasitic dinoflagellate Hematodinium, indicating that the NO/O-2(-)- generating and the antioxidant systems played vital roles in the crustacean innate immunity against the parasitic intrusion. The results indicated a novel respect of the host-parasite interaction between the crab host and the parasitic dinoflagellate Hematodinium. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrustacean Innate Immunity Parasites Nitric Oxide Synthase Nadph Oxidase Antioxidant Enzymes
DOI10.1016/j.fsi.2016.03.042
Indexed BySCI
Language英语
WOS IDWOS:000375165200028
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131032
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Meng,Wang, Jinfeng,Song, Shuqun,et al. Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium[J]. FISH & SHELLFISH IMMUNOLOGY,2016,52:263-277.
APA Li, Meng,Wang, Jinfeng,Song, Shuqun,&Li, Caiwen.(2016).Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium.FISH & SHELLFISH IMMUNOLOGY,52,263-277.
MLA Li, Meng,et al."Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles of NO/O-2(-)-generating and antioxidant systems in host immune responses to Hematodinium".FISH & SHELLFISH IMMUNOLOGY 52(2016):263-277.
Files in This Item:
File Name/Size DocType Version Access License
Molecular characteri(8413KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Meng]'s Articles
[Wang, Jinfeng]'s Articles
[Song, Shuqun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Meng]'s Articles
[Wang, Jinfeng]'s Articles
[Song, Shuqun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Meng]'s Articles
[Wang, Jinfeng]'s Articles
[Song, Shuqun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Molecular characterization of a novel nitric oxide synthase gene from Portunus trituberculatus and the roles.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.