| Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri | |
| Miao, Guoying; Qi, Haigang; Li, Li; Que, Huayong; Zhang, Guofan | |
| 2016-07-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 卷号 | 60页码:1-11 |
| 文章类型 | Article |
| 摘要 | The proteins of inhibitor of apoptosis (IAP) family play important roles in regulation of apoptosis, immunological response and cell proliferation. Here we reported two IAP genes (named CfIAP1 and CfIAP2) in Zhikong scallop Chlamys farreri. The full-length CfIAP1 cDNA contained 1552 nucleotides, encoding a predicted protein of 251 amino acids with two BIR domains. The full-length CfIAP2 cDNA contained 1243 nt, encoding a 356-aa protein with one BIR domain and one RING domain. The two genes are ubiquitously expressed in six types of tissue of C. farreri. The expression levels of CfIAP1 and CfIAP2 were significantly up-regulated after challenged with acute viral necrobiotic disease virus, lipopolysaccharide and exposure to air. Subcellular localization assay showed that CfIAP1 was mainly distributed in cytoplasm and CfIAP2 was in cytoplasm and nucleus. As assessed using a kit designed to test Caspase3 function in mammalian cells, the activity of CfCaspase3 was enhanced as a result of the down-regulation of CfIAP2 expression by dsRNA-mediated gene silencing. Our study indicated that CfIAP1 and CfIAP2 may participate in the innate immunity and stress responses and that CfIAP2 might block apoptosis via inhibiting CfCaspase3 indirectly through an unexplored mechanism in C. farreri. (C) 2016 Elsevier Ltd. All rights reserved. |
| 关键词 | Zhikong Scallop Chlamys Farreri Inhibitor Of Apoptosis Apoptosis Innate Immunity |
| DOI | 10.1016/j.dci.2016.02.012 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374613800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/131016 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Miao, Guoying,Qi, Haigang,Li, Li,et al. Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,60:1-11. |
| APA | Miao, Guoying,Qi, Haigang,Li, Li,Que, Huayong,&Zhang, Guofan.(2016).Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,60,1-11. |
| MLA | Miao, Guoying,et al."Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 60(2016):1-11. |
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| Characterization and（5265KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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