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Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri
Miao, Guoying; Qi, Haigang; Li, Li; Que, Huayong; Zhang, Guofan
2016-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume60Pages:1-11
SubtypeArticle
AbstractThe proteins of inhibitor of apoptosis (IAP) family play important roles in regulation of apoptosis, immunological response and cell proliferation. Here we reported two IAP genes (named CfIAP1 and CfIAP2) in Zhikong scallop Chlamys farreri. The full-length CfIAP1 cDNA contained 1552 nucleotides, encoding a predicted protein of 251 amino acids with two BIR domains. The full-length CfIAP2 cDNA contained 1243 nt, encoding a 356-aa protein with one BIR domain and one RING domain. The two genes are ubiquitously expressed in six types of tissue of C. farreri. The expression levels of CfIAP1 and CfIAP2 were significantly up-regulated after challenged with acute viral necrobiotic disease virus, lipopolysaccharide and exposure to air. Subcellular localization assay showed that CfIAP1 was mainly distributed in cytoplasm and CfIAP2 was in cytoplasm and nucleus. As assessed using a kit designed to test Caspase3 function in mammalian cells, the activity of CfCaspase3 was enhanced as a result of the down-regulation of CfIAP2 expression by dsRNA-mediated gene silencing. Our study indicated that CfIAP1 and CfIAP2 may participate in the innate immunity and stress responses and that CfIAP2 might block apoptosis via inhibiting CfCaspase3 indirectly through an unexplored mechanism in C. farreri. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordZhikong Scallop Chlamys Farreri Inhibitor Of Apoptosis Apoptosis Innate Immunity
DOI10.1016/j.dci.2016.02.012
Indexed BySCI
Language英语
WOS IDWOS:000374613800001
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131016
Collection海洋生物技术研发中心
AffiliationChinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Miao, Guoying,Qi, Haigang,Li, Li,et al. Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,60:1-11.
APA Miao, Guoying,Qi, Haigang,Li, Li,Que, Huayong,&Zhang, Guofan.(2016).Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,60,1-11.
MLA Miao, Guoying,et al."Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 60(2016):1-11.
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