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The characterization of hematopoietic tissue in adult Chinese mitten crab Eriocheir sinensis
Jia, Zhihao1,3; Kavungal, Sharath1,3; Jiang, Shuai1; Zhao, Depeng4; Sun, Mingzhe1,3; Wang, Lingling1; Song, Linsheng2
2016-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume60Pages:12-22
SubtypeArticle
AbstractInvertebrates rely on the efficient innate immune mechanisms against invaders, in which the continuous production of hemocytes (hematopoiesis) is indispensable. In the present study, the hematopoietic tissue (HPT) from Chinese mitten crab Eriocheir sinensis was identified and characterized. It was a thin and non transparent sheet located at the dorsolateral side of the stomach, which was composed of a series of ovoid lobules. Each lobule was surrounded by connective tissue containing a large amount of spherical cells with big nucleus. In HPT, the cells were full of mitochondria and granules, and DNA replication was detected in some cells by EdU labeling technique. Cell proliferation was observed in HPT by transmission electron microscope (TEM). The distribution of two transcription factors, GATA1 and RUNX1, were examined by human GATA1 and RUNX1 antibodies, respectively. Three homologues of RUNX1 were detected in the HPT while no signal of RUNX1 was observed in hemocytes, and GATA1 was detected in both HPT and some hemocytes. The mRNA transcript of a novel hematopoiesis related cytokine EsAst was detected in hepatopancreas and hemocytes, but it was no detectable in HPT. The mRNA expression level of EsAst in hepatopancreas was 1.38-fold higher than that in hemocytes. Total hemocytes counts were related to the mRNA expression level ofEsAst post Aeromonas hydrophila challenge. The results suggested that the stem cells in the hematopoietic tissue of Chinese mitten crab E. sinensis were regulated by transcriptional and humoral factors to generate hemocytes. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordEriocheir Sinensis Hematopoietic Tissue Morphology Histology Immunohistochemistry Edu
DOI10.1016/j.dci.2016.02.002
Indexed BySCI
Language英语
WOS IDWOS:000374613800002
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131013
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Polytech Univ, Dalian 116034, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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Jia, Zhihao,Kavungal, Sharath,Jiang, Shuai,et al. The characterization of hematopoietic tissue in adult Chinese mitten crab Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,60:12-22.
APA Jia, Zhihao.,Kavungal, Sharath.,Jiang, Shuai.,Zhao, Depeng.,Sun, Mingzhe.,...&Song, Linsheng.(2016).The characterization of hematopoietic tissue in adult Chinese mitten crab Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,60,12-22.
MLA Jia, Zhihao,et al."The characterization of hematopoietic tissue in adult Chinese mitten crab Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 60(2016):12-22.
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