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The modulation of haemolymph arginine kinase on the extracellular ATP induced bactericidal immune responses in the Pacific oyster Crassostrea gigas
Jiang, Shuai1; Jia, Zhihao1; Chen, Hao1; Wang, Lingling1; Song, Linsheng2
2016-07-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号54页码:282-293
文章类型Article
摘要Arginine kinase is an important phosphagen kinase (PK) which plays an essential role in ATP buffering systems in invertebrates. In the present study, an arginine kinase (designated CgAK) was isolated by the lipopolysaccharide (LPS) affinity chromatography from the haemolymph of Crassostrea gigas. CgAK could directly bind to LPS in a concentration-dependent manner with the dissociation constant (Kd) of 2.46 x 10(-6) M. The interaction with LPS significantly decreased the ATP hydrolytic activity of CgAK, which in turn lead to the accumulation of ATP in vitro. The extracellular ATP stimulation could induce Ca2+ influx, reactive oxygen species (ROS) production, and the release of lysosomal enzyme in the cellular immune response. In addition, ATP stimulation provoked the bactericidal activity towards Escherichia coli, and the scavenging ROS with N-acetyl-L-cysteine (NAC) abrogated the bactericidal activity, indicating that ATP stimulation could induce ROS-dependent antimicrobial activity in haemocytes. Collectively, the results demonstrated that the haemolymph CgAK could serve as an important purinergic regulator to modulate extracellular ATP, which might further have an important effect on the purinergic signaling-activated innate immune response of oyster. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Crassostrea Gigas Arginine Kinase Lipopolysaccharide Extracellular Atp Reactive Oxygen Species
DOI10.1016/j.fsi.2016.03.153
收录类别SCI
语种英语
WOS记录号WOS:000378451700034
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/131004
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
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Jiang, Shuai,Jia, Zhihao,Chen, Hao,et al. The modulation of haemolymph arginine kinase on the extracellular ATP induced bactericidal immune responses in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:282-293.
APA Jiang, Shuai,Jia, Zhihao,Chen, Hao,Wang, Lingling,&Song, Linsheng.(2016).The modulation of haemolymph arginine kinase on the extracellular ATP induced bactericidal immune responses in the Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,54,282-293.
MLA Jiang, Shuai,et al."The modulation of haemolymph arginine kinase on the extracellular ATP induced bactericidal immune responses in the Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 54(2016):282-293.
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