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Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization
Jiang, Likun1,2,5; You, Weiwei3; Zhang, Xiaojun4; Xu, Jian1,2; Jiang, Yanliang1,2; Wang, Kai1,2; Zhao, Zixia1,2; Chen, Baohua1,2,5; Zhao, Yunfeng1,2; Mahboob, Shahid6,7; Al-Ghanim, Khalid A.6; Ke, Caihuan3; Xu, Peng1,2
2016-02-01
Source PublicationMARINE BIOTECHNOLOGY
Volume18Issue:1Pages:49-56
SubtypeArticle
AbstractThe small abalone (Haliotis diversicolor) is one of the most important aquaculture species in East Asia. To facilitate gene cloning and characterization, genome analysis, and genetic breeding of it, we constructed a large-insert bacterial artificial chromosome (BAC) library, which is an important genetic tool for advanced genetics and genomics research. The small abalone BAC library includes 92,610 clones with an average insert size of 120 Kb, equivalent to approximately 7.6x of the small abalone genome. We set up three-dimensional pools and super pools of 18,432 BAC clones for target gene screening using PCR method. To assess the approach, we screened 12 target genes in these 18,432 BAC clones and identified 16 positive BAC clones. Eight positive BAC clones were then sequenced and assembled with the next generation sequencing platform. The assembled contigs representing these 8 BAC clones spanned 928 Kb of the small abalone genome, providing the first batch of genome sequences for genome evaluation and characterization. The average GC content of small abalone genome was estimated as 40.33 %. A total of 21 protein-coding genes, including 7 target genes, were annotated into the 8 BACs, which proved the feasibility of PCR screening approach with three-dimensional pools in small abalone BAC library. One hundred fifty microsatellite loci were also identified from the sequences for marker development in the future. The BAC library and clone pools provided valuable resources and tools for genetic breeding and conservation of H. diversicolor.
KeywordBac Small Abalone Haliotis Diversicolor Genome Ion Torrent
DOI10.1007/s10126-015-9666-4
Indexed BySCI
Language英语
WOS IDWOS:000378136600005
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131003
Collection实验海洋生物学重点实验室
海洋环流与波动重点实验室
Affiliation1.Chinese Acad Fishery Sci, CAFS Key Lab Aquat Genom, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China
2.Chinese Acad Fishery Sci, Beijing Key Lab Fishery Biotechnol, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China
3.Xiamen Univ, State Key Lab Marine Environm Sci, Coll Ocean & Earth Sci, Xiamen 361005, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
5.Shanghai Ocean Univ, Coll Life Sci, Shanghai 201306, Peoples R China
6.King Saud Univ, Coll Sci, Dept Zool, POB 2455, Riyadh 11451, Saudi Arabia
7.GC Univ, Dept Zool, Faisalabad, Pakistan
Recommended Citation
GB/T 7714
Jiang, Likun,You, Weiwei,Zhang, Xiaojun,et al. Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization[J]. MARINE BIOTECHNOLOGY,2016,18(1):49-56.
APA Jiang, Likun.,You, Weiwei.,Zhang, Xiaojun.,Xu, Jian.,Jiang, Yanliang.,...&Xu, Peng.(2016).Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization.MARINE BIOTECHNOLOGY,18(1),49-56.
MLA Jiang, Likun,et al."Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization".MARINE BIOTECHNOLOGY 18.1(2016):49-56.
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