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题名: Differentially proteomic analysis of the Chinese shrimp at WSSV latent and acute infection stages by iTRAQ approach
作者: Li, Shihao1,2; Li, Fuhua1,2; Sun, Zheng1; Zhang, Xiaojun1; Xiang, JianHai(相建海)1
刊名: FISH & SHELLFISH IMMUNOLOGY
出版日期: 2016-07-01
卷号: 54, 页码:629-638
关键词: WSSV ; Proteomics ; iTRAQ ; Fenneropenaeus chinensis ; Latent infection ; Acute infection ; Phagocytosis ; Apoptosis
DOI: 10.1016/j.fsi.2016.05.016
文章类型: Article
英文摘要: As the direct executors of biological function, the expression level of proteins will reveal the molecular mechanisms regulating WSSV acute infection more directly. In the present study, the iTRAQ approach was applied to identifying differentially expressed proteins in Chinese shrimp during WSSV latent infection and acute infection. A total of 4051 unique peptides corresponding to 1286 proteins were identified. 118 unique proteins showed differential up-regulation and 122 proteins were down-regulated in shrimp during WSSV acute infection compared with those in WSSV latent infection stage. A number of proteins related to actin-myosin cytoskeleton process, including myosin, actin, tubulin, clathrin, and tropomyosin were found up-regulated in shrimp at WSSV AI stage, indicating that the phagocytosis process was involved in WSSV AI stage. The apoptosis process in shrimp during WSSV AI seemed to be inhibited because some proteins suppressive on apoptosis were up-regulated, such as ALG-2 interacting protein x, Hsp90, 14-3-3-like protein, peroxiredoxin 5, peroxiredoxin 6 and adenine nucleotide translocase 2. Association analysis between the proteomic data and the previous transcriptome data was performed. Quantitative real-time PCR and western blot were carried out to verify the reliability of the proteomics data. The present study provided a comprehensive view of molecular mechanisms regulating WSSV acute infection at the protein level. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000378451700076
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内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.qdio.ac.cn/handle/337002/131001
Appears in Collections:实验海洋生物学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China

Recommended Citation:
Li, Shihao,Li, Fuhua,Sun, Zheng,et al. Differentially proteomic analysis of the Chinese shrimp at WSSV latent and acute infection stages by iTRAQ approach[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:629-638.
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