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Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells
Li, Meijie1,2,3; Tan, Xungang1,3; Sui, Yulei1,2,3; Jiao, Shuang1,3; Wu, Zhihao1,3; You, Feng1,3
2016-08-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume198Pages:66-72
SubtypeArticle
AbstractIn teleost fish, primordial germ cells (PGCs) are specified very early during embryogenesis and migrate to the site that gonads are formed. A previous study indicated that nanos3 is specifically expressed in PGCs, and the 3'untranslated region (UTR) of nanos3 is responsible for the localization of mRNA in these cells. In this study, we aimed to investigate the functional regions of nanos3 3'UTR in olive flounder using truncated and mutated nanos3 3'UTRs fused to chimeric RNAs and microinjected into fertilized zebrafish eggs. The results indicated that a 68-bp functional element in the nanos3 3'UTR of olive flounder played important roles in the protection and degradation of RNA. Within this element, a U-rich region was identified to be responsible for the protection of RNA in PGCs and two GCAC sites for the degradation of RNA in somatic cells. The first GCAC was located adjacently to the U-rich region and the second GCAC within the U-rich region. Overall, we concluded that the two GCACs were the binding sites of miR-430, a microRNA that suppresses translation, whereas the U-rich region was the binding site of Dnd, a protein that antagonizes the miR-430-mediated silencing of mRNA. (C)C. 2016 Elsevier Inc All rights reserved.
KeywordChimeric Rna Gcac Site Germ Plasm Microinjection U-rich Regions
DOI10.1016/j.cbpb.2016.04.002
Indexed BySCI
Language英语
WOS IDWOS:000378182300008
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/131000
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Meijie,Tan, Xungang,Sui, Yulei,et al. Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2016,198:66-72.
APA Li, Meijie,Tan, Xungang,Sui, Yulei,Jiao, Shuang,Wu, Zhihao,&You, Feng.(2016).Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,198,66-72.
MLA Li, Meijie,et al."Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 198(2016):66-72.
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