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Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells
Li, Meijie1,2,3; Tan, Xungang1,3; Sui, Yulei1,2,3; Jiao, Shuang1,3; Wu, Zhihao1,3; You, Feng1,3
2016-08-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷号198页码:66-72
文章类型Article
摘要In teleost fish, primordial germ cells (PGCs) are specified very early during embryogenesis and migrate to the site that gonads are formed. A previous study indicated that nanos3 is specifically expressed in PGCs, and the 3'untranslated region (UTR) of nanos3 is responsible for the localization of mRNA in these cells. In this study, we aimed to investigate the functional regions of nanos3 3'UTR in olive flounder using truncated and mutated nanos3 3'UTRs fused to chimeric RNAs and microinjected into fertilized zebrafish eggs. The results indicated that a 68-bp functional element in the nanos3 3'UTR of olive flounder played important roles in the protection and degradation of RNA. Within this element, a U-rich region was identified to be responsible for the protection of RNA in PGCs and two GCAC sites for the degradation of RNA in somatic cells. The first GCAC was located adjacently to the U-rich region and the second GCAC within the U-rich region. Overall, we concluded that the two GCACs were the binding sites of miR-430, a microRNA that suppresses translation, whereas the U-rich region was the binding site of Dnd, a protein that antagonizes the miR-430-mediated silencing of mRNA. (C)C. 2016 Elsevier Inc All rights reserved.
关键词Chimeric Rna Gcac Site Germ Plasm Microinjection U-rich Regions
DOI10.1016/j.cbpb.2016.04.002
收录类别SCI
语种英语
WOS记录号WOS:000378182300008
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/131000
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
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Li, Meijie,Tan, Xungang,Sui, Yulei,et al. Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2016,198:66-72.
APA Li, Meijie,Tan, Xungang,Sui, Yulei,Jiao, Shuang,Wu, Zhihao,&You, Feng.(2016).Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,198,66-72.
MLA Li, Meijie,et al."Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 198(2016):66-72.
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