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Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)
Liang, Zhongxiu1; Liu, Rui1; Zhao, Depeng3; Wang, Lingling2; Sun, Mingzhe1; Wang, Mengqiang1; Song, Linsheng2
2016-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume54Pages:523-528
SubtypeArticle
AbstractAmmonia is one of major environmental pollutants in the aquatic system that poses a great threat to the survival of shrimp. In the present study, the mRNA expression of endoplasmic reticulum (ER) stress marker and unfolded protein response (UPR) related genes, as well as the change of redox enzyme and apoptosis were investigated in hepatopancreas of the pacific white shrimp, Litopenaeus vannamei after the exposure of 20 mg L-1 total ammonia nitrogen (TAN). Compared with the control group, the superoxide dismutase (SOD) activity in hepatopancreas decreased significantly (p < 0.05) at 96 h, whereas the malonyldialdehyde (MDA) concentration increased significantly (p < 0.05). The mRNA expression levels of ER stress marker-immunoglobulin heavy chain binding protein (Bip) gene and key UPR related genes including activating transcription factor 4 (ATF4) and the spliced form of X box binding protein 1 (XBP1) increased significantly (p < 0.05) in hepatopancreas at 96 h after exposure to ammonia. In addition, apoptosis was observed obviously in the hepatopancreas of L. vannamei after exposure to ammonia by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. The results indicated that ammonia exposure could induce oxidative stress, which further caused ER stress and apoptosis in hepatopancreas of L. vannamei. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordLitopenaeus Vannamei Ammonia Exposure Oxidative Stress Endoplasmic Reticulum Stress Apoptosis
DOI10.1016/j.fsi.2016.05.009
Indexed BySCI
Language英语
WOS IDWOS:000378451700061
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130999
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture Stock Enhancement North China, Minist Agr, Dalian 116023, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liang, Zhongxiu,Liu, Rui,Zhao, Depeng,et al. Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:523-528.
APA Liang, Zhongxiu.,Liu, Rui.,Zhao, Depeng.,Wang, Lingling.,Sun, Mingzhe.,...&Song, Linsheng.(2016).Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei).FISH & SHELLFISH IMMUNOLOGY,54,523-528.
MLA Liang, Zhongxiu,et al."Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei)".FISH & SHELLFISH IMMUNOLOGY 54(2016):523-528.
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