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The Pacific White Shrimp beta-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus
Shi, Yingli1,2,3; Xiang, Jianhai1; Zhou, Guangzhou3,4; Ron, Tetsuzan Benny5; Tong, Hsin-I3; Kang, Wen3; Sun, Si3; Lu, Yuanan3
2016-06-01
Source PublicationMARINE BIOTECHNOLOGY
Volume18Issue:3Pages:349-358
SubtypeArticle
AbstractA newly isolated Pacific white shrimp (Litopenaeus vannamei) beta-actin promoter SbaP and its derivative compact construct SbaP (ENX) have recently been demonstrated to promote ectopic gene expression in vitro and in vivo. To further explore the potential transduction application, this newly isolated shrimp promoter SbaP was comparatively tested with cytomegalovirus (CMV), simian virus 40 (SV40), polyhedrin (Polh), and white spot syndrome virus immediate early gene 1 (WSSV ie1) four constitutive promoters and a beta-actin promoter (TbaP) from tilapia fish to characterize its promoting function in eight different cell lines. Luciferase quantitation assays revealed that SbaP can drive luciferase gene expression in all eight cell lines including sf21 (insect), PAC2 (zebrafish), EPC (carp), CHSE-214 (chinook salmon), GSTEF (green sea turtle), MS-1 (monk seal), 293T (human), and HeLa (human), but at different levels. Comparative analysis revealed that the promoting activity of SbaP was lower (<= 10-fold) than CMV but higher (2-20 folds) than Polh in most of these cell lines tested. Whereas, SbaP mediated luciferase expression in sf21 cells was over 20-fold higher than CMV, SV40, Polh, and TbaP promoter. Compared to the SbaP, SbaP (ENX), which was constructed on the basis of SbaP by deletion of two "negative" regulatory elements, exhibited no significant change of promoting activity in EPC and PAC2 cells, but a 5 and 16 % lower promoting effect in 293T and HeLa cells, respectively. Additionally, a recombinant baculovirus was constructed under the control of SbaP (ENX), and efficient promoter activity of newly generated baculoviral vector was detected both in vitro of infected sf21 cells and in vivo of injected indicator shrimp. These results warrant the potential application of SbaP, particularly SbaP (ENX) in ectopic gene expression in future.
KeywordThe Pacific White Shrimp Shrimp Beta-actin Promoter Sbap Promoter Activity Recombinant Baculovirus
DOI10.1007/s10126-016-9700-1
Indexed BySCI
Language英语
WOS IDWOS:000378136800006
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130996
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Hawaii Manoa, Dept Publ Hlth Sci, Honolulu, HI 96822 USA
4.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China
5.Univ Hawaii Manoa, Dept Human Nutr Food & Anim Sci, Honolulu, HI 96822 USA
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shi, Yingli,Xiang, Jianhai,Zhou, Guangzhou,et al. The Pacific White Shrimp beta-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus[J]. MARINE BIOTECHNOLOGY,2016,18(3):349-358.
APA Shi, Yingli.,Xiang, Jianhai.,Zhou, Guangzhou.,Ron, Tetsuzan Benny.,Tong, Hsin-I.,...&Lu, Yuanan.(2016).The Pacific White Shrimp beta-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus.MARINE BIOTECHNOLOGY,18(3),349-358.
MLA Shi, Yingli,et al."The Pacific White Shrimp beta-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus".MARINE BIOTECHNOLOGY 18.3(2016):349-358.
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