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The serine protease autotransporter Tsh contributes to the virulence of Edwardsiella tarda
Hu, Yong-hua1,2; Zhou, Hai-zhen1,3; Jin, Qian-wen1,3; Zhang, Jian1,2
2016-06-30
Source PublicationVETERINARY MICROBIOLOGY
Volume189Pages:68-74
SubtypeArticle
AbstractThe temperature-sensitive hemagglutinin (Tsh), identified as serine protease autotransporters of the Enterobacteriaceae (SPATEs) proteins, is an important virulence factor for avian-pathogenic Escherichia coli (APEC) and uropathogenic E. coli. However, little is known about the role of Tsh as a virulence factor in Edwardsiella tarda, a severe fish pathogen. In this study, we characterized the Tsh of E. tarda (named TshEt) and examined its function and vaccine potential. TshEt is composed of 1224 residues and has three functional domains typical for autotransporters. Quantitative real-time reverse transcriptase-PCR analysis showed that expression of tshEt was upregulated under conditions of high temperature, increased cell density, high pH, and iron starvation and during the infection of host cells. A markerless tsh in-frame mutant strain, TX01 Delta tsh, was constructed to determine whether TshEt participates in the pathogenicity of E. tarda, Compared to the wild type TX01, TX01 Delta tsh exhibited (i) retarded biofilm growth, (ii) decreased resistance against serum killing, (iii) impaired ability to block the host immune response, (iv) attenuated tissue and cellular infectivity. Introduction of a trans-expressed tsh gene restored the lost virulence of TX01 Delta tsh. The passenger domain of TshEt contains a putative serine protease (PepS) that exhibits apparent proteolytic activity when expressed in and purified from E. coli as a recombinant protein (rPepS). When used as a subunit vaccine to immunize Japanese flounder, rPepS was able to induce effective immune protection. This is the first study of Tsh in a fish pathogen, and the results suggest that TshEt exerts pleiotropic effects on the pathogenesis of E. tarda. (C) 2016 Elsevier B.V. All rights reserved.
KeywordAutotransporter Tsh Fish Pathogen Edwardsiella Tarda Virulence
DOI10.1016/j.vetmic.2016.04.021
Indexed BySCI
Language英语
WOS IDWOS:000378450900010
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130976
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Hu, Yong-hua,Zhou, Hai-zhen,Jin, Qian-wen,et al. The serine protease autotransporter Tsh contributes to the virulence of Edwardsiella tarda[J]. VETERINARY MICROBIOLOGY,2016,189:68-74.
APA Hu, Yong-hua,Zhou, Hai-zhen,Jin, Qian-wen,&Zhang, Jian.(2016).The serine protease autotransporter Tsh contributes to the virulence of Edwardsiella tarda.VETERINARY MICROBIOLOGY,189,68-74.
MLA Hu, Yong-hua,et al."The serine protease autotransporter Tsh contributes to the virulence of Edwardsiella tarda".VETERINARY MICROBIOLOGY 189(2016):68-74.
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