IOCAS-IR  > 实验海洋生物学重点实验室
A novel small heat shock protein of Haliotis discus hannai: characterization, structure modeling, and expression profiles under environmental stresses
Sun, Bo-guang1,2; Hu, Yong-hua1,2
2016-07-01
Source PublicationCELL STRESS & CHAPERONES
Volume21Issue:4Pages:583-591
SubtypeArticle
AbstractSmall heat shock proteins (sHsps) are a class of chaperones with low molecular weight, feathered by a C-terminal a-crystallin domain (ACD). They participate in reestablishing the stability of partially denatured proteins and therefore contribute to cellular homeostasis. In this work, we identified a sHsp homolog (designated as sHsp19) from Haliotis discus hannai, an economically important farmed mollusk in East Asia. sHsp19 possesses a sHsp hallmark domain, which exhibits the typical fold of ACD as revealed by a three-dimensional model constructed through an iterative threading assembly refinement method. The amino acid sequence sHsp19 shares low identities with any other known sHsps, with percentages below 35 %. Besides, sHsp19 shows relatively distant phylogenetic relationships with sHsps of various mollusks, including two other identified sHsps of abalone subspecies. qRT-PCR analysis indicated that the expression of sHsp19 occurred in multiple tissues. Upon exposure to thermal, oxidative, and multiple toxic metal stresses, the level of sHsp19 mRNA was rapidly elevated in a persistent fashion, with the maximum increase up to 170.58-, 405.84-, and 361.96-fold, respectively. These results indicate sHsp is a novel sHsp that possesses the distinguishing structural feature of sHsps but has remote homologies with known sHsps. It is likely to be important in stress adaptation of abalone and may be applied as a bioindicator for monitoring pollution or detrimental changes of environment in abalone culture.
KeywordMollusk Abalone Shsp Environmental Stress Bioindicator
DOI10.1007/s12192-016-0683-7
Indexed BySCI
Language英语
WOS IDWOS:000378579800005
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130973
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Bo-guang,Hu, Yong-hua. A novel small heat shock protein of Haliotis discus hannai: characterization, structure modeling, and expression profiles under environmental stresses[J]. CELL STRESS & CHAPERONES,2016,21(4):583-591.
APA Sun, Bo-guang,&Hu, Yong-hua.(2016).A novel small heat shock protein of Haliotis discus hannai: characterization, structure modeling, and expression profiles under environmental stresses.CELL STRESS & CHAPERONES,21(4),583-591.
MLA Sun, Bo-guang,et al."A novel small heat shock protein of Haliotis discus hannai: characterization, structure modeling, and expression profiles under environmental stresses".CELL STRESS & CHAPERONES 21.4(2016):583-591.
Files in This Item:
File Name/Size DocType Version Access License
A novel small heat s(1085KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Bo-guang]'s Articles
[Hu, Yong-hua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Bo-guang]'s Articles
[Hu, Yong-hua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Bo-guang]'s Articles
[Hu, Yong-hua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A novel small heat shock protein of Haliotis discus hannai_ characterization, structure modeling, and expression profiles under environmental stresses.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.