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Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis; Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis
Liu, Chenlin1,2; Wang, Xiuliang2,3; Wang, Xingna1; Sun, Chengjun1
2016-07-01 ; 2016-07-01
Source PublicationEXTREMOPHILES ; EXTREMOPHILES
Volume20Issue:4Pages:437-450
SubtypeArticle ; Article
AbstractThe Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the sea-ice brine, such as freezing temperature and high salinity. We performed a transcriptome analysis to identify freezing stress responding genes and explore the extreme environmental acclimation-related strategies. Here, we show that many genes in ICE-L transcriptome that encoding PUFA synthesis enzymes, molecular chaperon proteins, and cell membrane transport proteins have high similarity to the gens from Antarctic bacteria. These ICE-L genes are supposed to be acquired through horizontal gene transfer from its symbiotic microbes in the sea-ice brine. The presence of these genes in both sea-ice microalgae and bacteria indicated the biological processes they involved in are possibly contributing to ICE-L success in sea ice. In addition, the biological pathways were compared between ICE-L and its closely related sister species, Chlamydomonas reinhardtii and Volvox carteri. In ICE-L transcripome, many sequences homologous to the plant or bacteria proteins in the post-transcriptional, post-translational modification, and signal-transduction KEGG pathways, are absent in the nonpsychrophilic green algae. These complex structural components might imply enhanced stress adaptation capacity. At last, differential gene expression analysis at the transcriptome level of ICE-L indicated that genes that associated with post-translational modification, lipid metabolism, and nitrogen metabolism are responding to the freezing treatment. In conclusion, the transcriptome of Chlamydomonas sp. ICE-L is very useful for exploring the mutualistic interaction between microalgae and bacteria in sea ice; and discovering the specific genes and metabolism pathways responding to the freezing acclimation in psychrophilic microalgae.; The Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the sea-ice brine, such as freezing temperature and high salinity. We performed a transcriptome analysis to identify freezing stress responding genes and explore the extreme environmental acclimation-related strategies. Here, we show that many genes in ICE-L transcriptome that encoding PUFA synthesis enzymes, molecular chaperon proteins, and cell membrane transport proteins have high similarity to the gens from Antarctic bacteria. These ICE-L genes are supposed to be acquired through horizontal gene transfer from its symbiotic microbes in the sea-ice brine. The presence of these genes in both sea-ice microalgae and bacteria indicated the biological processes they involved in are possibly contributing to ICE-L success in sea ice. In addition, the biological pathways were compared between ICE-L and its closely related sister species, Chlamydomonas reinhardtii and Volvox carteri. In ICE-L transcripome, many sequences homologous to the plant or bacteria proteins in the post-transcriptional, post-translational modification, and signal-transduction KEGG pathways, are absent in the nonpsychrophilic green algae. These complex structural components might imply enhanced stress adaptation capacity. At last, differential gene expression analysis at the transcriptome level of ICE-L indicated that genes that associated with post-translational modification, lipid metabolism, and nitrogen metabolism are responding to the freezing treatment. In conclusion, the transcriptome of Chlamydomonas sp. ICE-L is very useful for exploring the mutualistic interaction between microalgae and bacteria in sea ice; and discovering the specific genes and metabolism pathways responding to the freezing acclimation in psychrophilic microalgae.
KeywordAntarctic Sea Ice Antarctic Sea Ice Freezing Acclimation Freezing Acclimation Horizontal Gene Transfer Horizontal Gene Transfer Psychrophilic Green Microalga Psychrophilic Green Microalga Transcriptomic Analysis Transcriptomic Analysis
DOI10.1007/s00792-016-0834-x ; 10.1007/s00792-016-0834-x
Indexed BySCI ; SCI
Language英语 ; 英语
WOS IDWOS:000379027600007 ; WOS:000379027600007
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130964
Collection实验海洋生物学重点实验室
Affiliation1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Xianxialing Rd 6th, Qingdao 266061, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Liu, Chenlin,Wang, Xiuliang,Wang, Xingna,et al. Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis, Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis[J]. EXTREMOPHILES, EXTREMOPHILES,2016, 2016,20, 20(4):437-450, 437-450.
APA Liu, Chenlin,Wang, Xiuliang,Wang, Xingna,&Sun, Chengjun.(2016).Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis.EXTREMOPHILES,20(4),437-450.
MLA Liu, Chenlin,et al."Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis".EXTREMOPHILES 20.4(2016):437-450.
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