IOCAS-IR  > 实验海洋生物学重点实验室
Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival
Zhou, Ze-jun1,2,3; Sun, Li1,2
2016-07-14
发表期刊FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷号6
文章类型Article
摘要Edwardsiella tarda is a Gram-negative bacterial pathogen that can infect a wide range of freshwater and marine fish. One salient feature of E. tarda is the ability to survive and replicate in various host cells. In this study, we observed that E. tarda replicated robustly in the zebrafish cell line ZF4, and that E tarda-infected cells exhibited no detectable signs of apoptosis. Global transcriptome analysis and quantitative real-time RT-PCR revealed that E. tarda infection generally significantly downregulated pro-apoptotic genes and upregulated anti-apoptotic genes. To investigate the role of apoptosis in E. tarda infection, two upregulated anti-apoptotic genes (Fech and Prx3) and two downregulated pro-apoptotic genes (Brms1a and Ivns1a) were overexpressed in zebrafish. Subsequent infection study showed that Fech and Prx3 overexpression significantly promoted E. tarda dissemination in and colonization of fish tissues, while Brms1a and Ivns1a overexpression significantly reduced E tarda dissemination and colonization. Consistently, when Fech and Prx3 were knocked down in zebrafish, E. tarda infection was significantly inhibited, whereas Brms1a and Ivns1a knockdown significantly enhanced E. tarda infection. These results indicate for the first time that E. tarda prevents apoptosis in teleost as a strategy for intracellular survival, and that some putative apoptotic genes of teleost function in the apoptosis pathway probably in a manner similar to that in mammalian systems.
关键词Edwardsiella Tarda Invasion Intracellular Replication Apoptosis Zebrafish
DOI10.3389/fcimb.2016.00076
收录类别SCI
语种英语
WOS记录号WOS:000379655500001
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/130952
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Ze-jun,Sun, Li. Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2016,6.
APA Zhou, Ze-jun,&Sun, Li.(2016).Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,6.
MLA Zhou, Ze-jun,et al."Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 6(2016).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Edwardsiella tarda-I(1632KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhou, Ze-jun]的文章
[Sun, Li]的文章
百度学术
百度学术中相似的文章
[Zhou, Ze-jun]的文章
[Sun, Li]的文章
必应学术
必应学术中相似的文章
[Zhou, Ze-jun]的文章
[Sun, Li]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Edwardsiella tarda-Induced Inhibition of Apoptosis_ A Strategy for Intracellular Survival.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。