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Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy
Yang, Haoyue1,2; Xing, Ronge1; Hu, Linfeng1; Liu, Song1; Li, Pengcheng1
2016-07-01
Source PublicationMICROBIAL BIOTECHNOLOGY
Volume9Issue:4Pages:478-485
SubtypeArticle
AbstractIn this study, a new bacterial strain having a high ability to produce -aminobutyric acid (GABA) was isolated from naturally fermented scallop solution and was identified as Enterococcus avium. To the best of our knowledge, this is the first study to prove that E.avium possesses glutamate decarboxylase activity. The strain was then mutagenized with UV radiation and was designated as E.avium 9184. Scallop solution was used as the culture medium to produce GABA. A two-stage fermentation strategy was applied to accumulate GABA. In the first stage, cell growth was regulated. Optimum conditions for cell growth were pH, 6.5; temperature, 37 degrees C; and glucose concentration, 10gL(-1). This produced a maximum dry cell mass of 2.10gL(-1). In the second stage, GABA formation was regulated. GABA concentration reached 3.71gL(-1) at 96h pH 6.0, 37 degrees C and initial l-monosodium glutamate concentration of 10gL(-1). Thus, compared with traditional one-stage fermentation, the two-stage fermentation significantly increased GABA accumulation. These results provide preliminary data to produce GABA using E.avium and also provide a new approach to process and utilize shellfish.
DOI10.1111/1751-7915.12301
Indexed BySCI
Language英语
WOS IDWOS:000379689900006
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130947
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, Haoyue,Xing, Ronge,Hu, Linfeng,et al. Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy[J]. MICROBIAL BIOTECHNOLOGY,2016,9(4):478-485.
APA Yang, Haoyue,Xing, Ronge,Hu, Linfeng,Liu, Song,&Li, Pengcheng.(2016).Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy.MICROBIAL BIOTECHNOLOGY,9(4),478-485.
MLA Yang, Haoyue,et al."Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy".MICROBIAL BIOTECHNOLOGY 9.4(2016):478-485.
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