| Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy | |
Yang, Haoyue1,2 ; Xing, Ronge1; Hu, Linfeng1; Liu, Song1; Li, Pengcheng1
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| 2016-07-01 | |
| 发表期刊 | MICROBIAL BIOTECHNOLOGY
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| 卷号 | 9期号:4页码:478-485 |
| 文章类型 | Article |
| 摘要 | In this study, a new bacterial strain having a high ability to produce -aminobutyric acid (GABA) was isolated from naturally fermented scallop solution and was identified as Enterococcus avium. To the best of our knowledge, this is the first study to prove that E.avium possesses glutamate decarboxylase activity. The strain was then mutagenized with UV radiation and was designated as E.avium 9184. Scallop solution was used as the culture medium to produce GABA. A two-stage fermentation strategy was applied to accumulate GABA. In the first stage, cell growth was regulated. Optimum conditions for cell growth were pH, 6.5; temperature, 37 degrees C; and glucose concentration, 10gL(-1). This produced a maximum dry cell mass of 2.10gL(-1). In the second stage, GABA formation was regulated. GABA concentration reached 3.71gL(-1) at 96h pH 6.0, 37 degrees C and initial l-monosodium glutamate concentration of 10gL(-1). Thus, compared with traditional one-stage fermentation, the two-stage fermentation significantly increased GABA accumulation. These results provide preliminary data to produce GABA using E.avium and also provide a new approach to process and utilize shellfish. |
| DOI | 10.1111/1751-7915.12301 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000379689900006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/130947 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Yang, Haoyue,Xing, Ronge,Hu, Linfeng,et al. Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy[J]. MICROBIAL BIOTECHNOLOGY,2016,9(4):478-485. |
| APA | Yang, Haoyue,Xing, Ronge,Hu, Linfeng,Liu, Song,&Li, Pengcheng.(2016).Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy.MICROBIAL BIOTECHNOLOGY,9(4),478-485. |
| MLA | Yang, Haoyue,et al."Accumulation of -aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy".MICROBIAL BIOTECHNOLOGY 9.4(2016):478-485. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Accumulation of -ami（1849KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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