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Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus
Peng Limin1,2,3; Zheng Yuan1,2; You Feng1,2; Wu Zhihao1,2; Zou Yuxia1,2; Zhang Peijun1,2
2016-09-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume34Issue:5Pages:1054-1063
SubtypeArticle
AbstractThe culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25 degrees C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical "S" shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.
KeywordOlive Flounder Paralichthys Olivaceus Testis Sertoli Cell Chromosome Fasl Transfection
DOI10.1007/s00343-016-5091-4
Indexed BySCI
Language英语
WOS IDWOS:000379737000017
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130938
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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Peng Limin,Zheng Yuan,You Feng,et al. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1054-1063.
APA Peng Limin,Zheng Yuan,You Feng,Wu Zhihao,Zou Yuxia,&Zhang Peijun.(2016).Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1054-1063.
MLA Peng Limin,et al."Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1054-1063.
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