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Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus
Peng Limin1,2,3; Zheng Yuan1,2; You Feng1,2; Wu Zhihao1,2; Zou Yuxia1,2; Zhang Peijun1,2
2016-09-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号34期号:5页码:1054-1063
文章类型Article
摘要The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25 degrees C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical "S" shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.
关键词Olive Flounder Paralichthys Olivaceus Testis Sertoli Cell Chromosome Fasl Transfection
DOI10.1007/s00343-016-5091-4
收录类别SCI
语种英语
WOS记录号WOS:000379737000017
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/130938
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Peng Limin,Zheng Yuan,You Feng,et al. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1054-1063.
APA Peng Limin,Zheng Yuan,You Feng,Wu Zhihao,Zou Yuxia,&Zhang Peijun.(2016).Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1054-1063.
MLA Peng Limin,et al."Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1054-1063.
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