IOCAS-IR  > 实验海洋生物学重点实验室
Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (Paralichthys olivaceus)
Weng, Shenda1,2; You, Feng1,3; Fan, Zhaofei1,2; Wang, Lijuan1,2; Wu, Zhihao1,3; Zou, Yuxia1,3
2016-08-01
发表期刊FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号42期号:4页码:1167-1176
文章类型Article
摘要WNT4 (wingless-type MMTV integration site family, member 4) is regarded as a key regulator of gonad differentiation in mammalians. However, the potential role of wnt4 in teleosts during gonad differentiation and development is still unclear. The full-length cDNA sequence of wnt4 in olive flounder (Paralichthys olivaceus) was obtained using RACE (rapid amplification of cDNA ends) technique. The wnt4 ORF contains 1059 nucleotides, encoding a protein with a signal peptide domain and a wnt family domain. Expression in tissues of adult flounders was analyzed by real-time RT-PCR. The results showed that wnt4 was widely expressed in multiple tissues of flounders, and the expression level was significantly higher in ovary than in testis. Then wnt4 expression pattern was investigated during gonadal differentiation period and at gonadal development stages (I-V). The results showed the expression levels were significantly higher in testis than in ovary during gonadal differentiation. Notably, wnt4 expression had a very significant increase before testis differentiation. At gonad different developmental stages, there was no expression signal at stage I or stage II, and the expression of wnt4 was much stronger in ovary than in testis at stage III and stage IV, followed by a faint expression in stage V in both sexes. Our results imply that cloned wnt4 could be wnt4a. It is a sex-related gene and its expression pattern in gonadal differentiation period of flounder is different from that in mammalians or other teleosts. Flounder wnt4 might play more important role in testis than in ovary during gonadal differentiation.
关键词Olive Flounder Wnt4 Molecular Cloning Sexually Dimorphic Expression Gonadal Differentiation Gonadal Development
DOI10.1007/s10695-016-0206-6
收录类别SCI
语种英语
WOS记录号WOS:000379734500009
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/130937
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Weng, Shenda,You, Feng,Fan, Zhaofei,et al. Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (Paralichthys olivaceus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2016,42(4):1167-1176.
APA Weng, Shenda,You, Feng,Fan, Zhaofei,Wang, Lijuan,Wu, Zhihao,&Zou, Yuxia.(2016).Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (Paralichthys olivaceus).FISH PHYSIOLOGY AND BIOCHEMISTRY,42(4),1167-1176.
MLA Weng, Shenda,et al."Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (Paralichthys olivaceus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 42.4(2016):1167-1176.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Molecular cloning an(1728KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Weng, Shenda]的文章
[You, Feng]的文章
[Fan, Zhaofei]的文章
百度学术
百度学术中相似的文章
[Weng, Shenda]的文章
[You, Feng]的文章
[Fan, Zhaofei]的文章
必应学术
必应学术中相似的文章
[Weng, Shenda]的文章
[You, Feng]的文章
[Fan, Zhaofei]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Molecular cloning and sexually dimorphic expression of wnt4 in olive .pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。