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The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)
Liu, Conghui1,3; Zhang, Tao1,3; Wang, Lingling1; Wang, Mengqiang1; Wang, Weilin1,3; Jia, Zhihao1,3; Jiang, Shuai1; Song, Linsheng2
2016-10-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume63Pages:163-170
SubtypeArticle
AbstractExtracellular superoxide dismutase (EcSOD) is a copper-containing glycoprotein playing an important role in antioxidant defense of living cells exposed to oxidative stress, and also participating in microorganism internalization and cell adhesion in invertebrates. EcSOD from oyster (designated CgEcSOD) had been previously reported to bind lipopolysaccharides (LPS) and act as a bridge molecule in Vibrio splendidus internalization. Its mRNA expression pattern, PAMP binding spectrum and microorganism binding capability were examined in the present study. The mRNA expression of CgEcSOD in hemocytes was significantly up-regulated at the initial phase and decreased sharply at 48 h post V. splendidus stimulation. The recombinant CgEcSOD protein (rCgEcSOD) could bind LPS, PGN and poly (I:C), as well as various microorganisms including Micrococcus luteus, Staphylococcus aureus, Escherichia coli, Vibrio anguillarum, V splendidus, Pastoris pastoris and Yarrowia lipolytica at the presence of divalent metal ions Cu2+. After the secondary V. splendidus stimulation, the mRNA and protein of CgEcSOD were both down regulated significantly. The results collectively indicated that CgEcSOD could not only function in the immune recognition, but also might contribute to the immune priming of oyster by inhibiting the foreign microbe invasion through a specific down-regulation. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordExtracellular Superoxide Dismutase Rgd Motif Crassostrea Gigas Inhibiting Factor Immune Recognition Immune Priming
DOI10.1016/j.dci.2016.06.002
Indexed BySCI
Language英语
WOS IDWOS:000380623300018
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130891
Collection实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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Liu, Conghui,Zhang, Tao,Wang, Lingling,et al. The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,63:163-170.
APA Liu, Conghui.,Zhang, Tao.,Wang, Lingling.,Wang, Mengqiang.,Wang, Weilin.,...&Song, Linsheng.(2016).The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,63,163-170.
MLA Liu, Conghui,et al."The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 63(2016):163-170.
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