IOCAS-IR  > 实验海洋生物学重点实验室
Identification and function analysis of a novel vascular endothelial growth factor, LvVEGF3, in the Pacific whiteleg shrimp Litopenaeus vannamei
Wang, Zhiwei1,2; Li, Shihao1,3; Li, Fuhua1,3; Xie, Shijun1,2; Xiang, Jianhai1,3
2016-10-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume63Pages:111-120
SubtypeArticle
AbstractVEGF signaling pathway is first discovered in mammals and proved to play important roles in the biological processes of angiogenesis, tumor migration, cell differentiation, apoptosis, host-virus interaction etc. Three members in the VEGF signaling pathway, including LvVEGFR, LvVEGF1 and LvVEGF2 in shrimp have been proved to be related with WSSV infection in our previous studies. Currently, another member of VEGF family, LvVEGF3, was isolated and its function during the WSSV infection of shrimp was studied. The deduced amino acid sequence of LvVEGF3 contained a signal peptide, a typical PDGF/VEGF domain and a cysteine-knot motif (CXCXC). Tissue distribution analysis showed that LvVEGF3 was predominantly expressed in hemocytes. The transcriptional level of LvVEGF3 in hemocytes was apparently up-regulated during WSSV infection. Silencing of LvVEGF3 with double-stranded RNA caused a reduction of the cumulative mortality rate of shrimp during WSSV infection. The expression of LvVEGFR was apparently down-regulated after LvVEGF3 silencing and up-regulated after injection of recombinant LvVEGF3 protein, suggesting an interaction between LvVEGF3 and LvVEGFR. Furthermore, the interaction between LvVEGFR and LvVEGF3 was confirmed using the yeast two-hybrid system. The results provided new insights into understanding the role of VEGF signaling pathway during virus infection. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordVascular Endothelial Growth Factor Litopenaeus Vannamei Wssv Rna Interference Interaction
DOI10.1016/j.dci.2016.05.020
Indexed BySCI
Language英语
WOS IDWOS:000380623300012
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/130890
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl & Local Joint Engn Lab Ecol Mariculture, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Zhiwei,Li, Shihao,Li, Fuhua,et al. Identification and function analysis of a novel vascular endothelial growth factor, LvVEGF3, in the Pacific whiteleg shrimp Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,63:111-120.
APA Wang, Zhiwei,Li, Shihao,Li, Fuhua,Xie, Shijun,&Xiang, Jianhai.(2016).Identification and function analysis of a novel vascular endothelial growth factor, LvVEGF3, in the Pacific whiteleg shrimp Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,63,111-120.
MLA Wang, Zhiwei,et al."Identification and function analysis of a novel vascular endothelial growth factor, LvVEGF3, in the Pacific whiteleg shrimp Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 63(2016):111-120.
Files in This Item:
File Name/Size DocType Version Access License
Identification and f(2040KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Zhiwei]'s Articles
[Li, Shihao]'s Articles
[Li, Fuhua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Zhiwei]'s Articles
[Li, Shihao]'s Articles
[Li, Fuhua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Zhiwei]'s Articles
[Li, Shihao]'s Articles
[Li, Fuhua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Identification and function analysis of a novel vascular endothelial growth factor, LvVEGF3, in the Pacific whiteleg shrimp Litopenaeus vannamei.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.