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Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination
Wang, Yujuan1; Wang, Xiuhua1,2; Huang, Jie1,2; Li, Jun2,3,4
2016-03-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume17Issue:3
SubtypeArticle
AbstractThe adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 +/- 13.6)%, (81.7 +/- 8.2)%, (77.8 +/- 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route.
KeywordVaccination Relative Percent Of Survival (Rps) Quillaja Saponaria Saponins Scophthalmus Maximus Adjuvant
DOI10.3390/ijms17030325
Indexed BySCI
Language英语
WOS IDWOS:000373712800078
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/126271
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
4.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA
Recommended Citation
GB/T 7714
Wang, Yujuan,Wang, Xiuhua,Huang, Jie,et al. Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3).
APA Wang, Yujuan,Wang, Xiuhua,Huang, Jie,&Li, Jun.(2016).Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(3).
MLA Wang, Yujuan,et al."Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.3(2016).
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