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Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition
Wang, Mengqiang1; Wang, Lingling1; Xin, Lusheng1,3; Wang, Xiudan1,3; Wang, Lin1,3; Xu, Jianchao1,3; Jia, Zhihao1,3; Yue, Feng1; Wang, Hao1; Song, Linsheng2
2016-06-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume59Pages:99-109
SubtypeArticle
AbstractLeucine-rich repeat (LRR)-only proteins could mediate protein-ligand and protein protein interactions and be involved in the immune response. In the present study, two novel LRR-only proteins, CfLRRop-2 and CfLRRop-3, were identified and characterized from scallop Chlamys farreri. They both contained nine LRR motifs with the consensus signature sequence LxxLxLxxNxL and formed typical horseshoe structure. The CfLRRop-2 and CfLRRop-3 mRNA transcripts were constitutively expressed in haemocytes, muscle, mantle, gill, haepatopancreas and gonad, with the highest expression level in haepatopancreas and gill, respectively. During the ontogenesis of scallop, the mRNA transcripts of CfLRRop-2 were kept at a high level in oocytes and embryos, while those of CfLRRop-3 were expressed at a rather low level from oocytes to blastula. Their mRNA transcripts were significantly increased after the stimulation of lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C), and the mRNA expression of CfLRRop-2 rose more intensely than that of CfLRRop-3. After the suppression of CfTLR (previously identified Toll-like receptor in C. farreri) via RNA interference (RNAi), CfLRRop-3 mRNA transcripts increased more intensely and lastingly than those of CfLRRop-2. The rCfLRRop-3 protein could bind LPS, PGN, GLU and poly I:C, while rCfLRRop-2 exhibited no significant binding activity to them. Additionally, rCfLRRop-2 could significantly induce the release of TNF-alpha from the mixed primary cultured scallop haemocytes, but rCfLRRop-3 failed. These results collectively indicated that CfLRRop-2 might act as an immune effector or pro-inflammatory factor, while CfLRRop-3 would function as a pattern recognition receptor (PRR), suggesting the function of LRR-only protein family has differentiated in scallop. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordChlamys Farreri Innate Immunity Leucine-rich Repeat Lrr-only Protein Recognition Pattern
DOI10.1016/j.dci.2016.01.013
Indexed BySCI
Language英语
WOS IDWOS:000373547000011
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/126257
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
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GB/T 7714
Wang, Mengqiang,Wang, Lingling,Xin, Lusheng,et al. Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,59:99-109.
APA Wang, Mengqiang.,Wang, Lingling.,Xin, Lusheng.,Wang, Xiudan.,Wang, Lin.,...&Song, Linsheng.(2016).Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,59,99-109.
MLA Wang, Mengqiang,et al."Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 59(2016):99-109.
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