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Development of a 3-hydroxypropionate resistant Escherichia coli strain
Liu, Min1,2; Han, Xueping1,2; Xian, Mo1; Ding, Yamei3; Liu, Huizhou1; Zhao, Guang1
2016
Source PublicationBIOENGINEERED
Volume7Issue:1Pages:21-27
SubtypeArticle
Abstract3-hydroxypropionate (3HP) is an important platform chemical, and its biosynthesis is severely restricted by the toxicity of 3HP on cell growth and survival. To improve Escherichia coli resistance to 3HP and reduce the total production cost in industrial applications, we have identified variations in protein expression level exposed to sub-lethal concentration of this chemical using 2-dimensional gel electrophoresis. Under 3HP stress, the amount of 46 proteins was increased while the amount of 23 proteins was reduced. According to the proteomic results, overexpression of some identified proteins significantly increased the E. coli survival rate under 3HP stress. This study shed light on clues for developing E. coli strains with higher resistance to 3HP toxicity and lower production cost for industrial applications.
KeywordBacterial Stress Response Comparative Proteomics Escherichia Coli 3-hydroxypropionate 3-hydroxypropionate Resistance
DOI10.1080/21655979.2015.1122143
Indexed BySCI
Language英语
WOS IDWOS:000373159100009
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/126243
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Liu, Min,Han, Xueping,Xian, Mo,et al. Development of a 3-hydroxypropionate resistant Escherichia coli strain[J]. BIOENGINEERED,2016,7(1):21-27.
APA Liu, Min,Han, Xueping,Xian, Mo,Ding, Yamei,Liu, Huizhou,&Zhao, Guang.(2016).Development of a 3-hydroxypropionate resistant Escherichia coli strain.BIOENGINEERED,7(1),21-27.
MLA Liu, Min,et al."Development of a 3-hydroxypropionate resistant Escherichia coli strain".BIOENGINEERED 7.1(2016):21-27.
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