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题名: Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)
作者: Liu, Shilin1; Zhou, Yi(周毅)1; Zhang, Tao(张涛)1; Sun, Lina1; Ru, Xiaoshang1; Li, Junhua2; Yang, HongSheng(杨红生)1
刊名: INVERTEBRATE REPRODUCTION & DEVELOPMENT
出版日期: 2016-06-01
卷号: 60, 期号:2, 页码:131-136
关键词: Apostichopus japonicus ; oocyte ; GVBD ; drying ; development
DOI: 10.1080/07924259.2016.1174154
文章类型: Article
英文摘要: Techniques for in vitro oocyte maturation are important for studying maturation regulatory mechanisms, cell differentiation, and embryonic development. In this study, after Apostichopus japonicus gonads were dissected, oocytes, together with gonadal fluid extracted from gonads, were dried in shade. The effects of three temperatures (17, 21, and 25 degrees C) on oocyte maturation and following development were studied. The results showed that germinal vesicle breakdown (GVBD) of most oocytes occurred after desiccation, and those oocytes with GVBD could be fertilized and develop normally. There were no significant differences in the percentages of GVBD and individuals in blastula and gastrula stages between 17 and 21 degrees C (p > .05). The percentages of GVBD reached a maximum (17 degrees C: 98.6%, 21 degrees C: 97.1%) after 2 h of desiccation. The percentages of blastulae and gastrulae reached the maximum of more than 80% after 2.5-3.5 h of desiccation. At 25 degrees C, the percentages of GVBD, blastulae, and gastrulae reached a maximum of 76.7, 61.0, and 59.9% after 3.5 h of desiccation, respectively, and were significantly lower than that at 17 and 21 degrees C (p < .01). This study not only provided an effective and simple technique for in vitro induction of A. japonicus oocyte maturation, but also furthered understanding of gamete biology of this species.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000377424500007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.qdio.ac.cn/handle/337002/126241
Appears in Collections:海洋生态与环境科学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai, Peoples R China

Recommended Citation:
Liu, Shilin,Zhou, Yi,Zhang, Tao,et al. Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT,2016,60(2):131-136.
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文件名: Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus .pdf
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