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Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)
Liu, Shilin1; Zhou, Yi1; Zhang, Tao1; Sun, Lina1; Ru, Xiaoshang1; Li, Junhua2; Yang, Hongsheng1
2016-06-01
发表期刊INVERTEBRATE REPRODUCTION & DEVELOPMENT
卷号60期号:2页码:131-136
文章类型Article
摘要Techniques for in vitro oocyte maturation are important for studying maturation regulatory mechanisms, cell differentiation, and embryonic development. In this study, after Apostichopus japonicus gonads were dissected, oocytes, together with gonadal fluid extracted from gonads, were dried in shade. The effects of three temperatures (17, 21, and 25 degrees C) on oocyte maturation and following development were studied. The results showed that germinal vesicle breakdown (GVBD) of most oocytes occurred after desiccation, and those oocytes with GVBD could be fertilized and develop normally. There were no significant differences in the percentages of GVBD and individuals in blastula and gastrula stages between 17 and 21 degrees C (p > .05). The percentages of GVBD reached a maximum (17 degrees C: 98.6%, 21 degrees C: 97.1%) after 2 h of desiccation. The percentages of blastulae and gastrulae reached the maximum of more than 80% after 2.5-3.5 h of desiccation. At 25 degrees C, the percentages of GVBD, blastulae, and gastrulae reached a maximum of 76.7, 61.0, and 59.9% after 3.5 h of desiccation, respectively, and were significantly lower than that at 17 and 21 degrees C (p < .01). This study not only provided an effective and simple technique for in vitro induction of A. japonicus oocyte maturation, but also furthered understanding of gamete biology of this species.
关键词Apostichopus Japonicus Oocyte Gvbd Drying Development
DOI10.1080/07924259.2016.1174154
收录类别SCI
语种英语
WOS记录号WOS:000377424500007
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/126241
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai, Peoples R China
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GB/T 7714
Liu, Shilin,Zhou, Yi,Zhang, Tao,et al. Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT,2016,60(2):131-136.
APA Liu, Shilin.,Zhou, Yi.,Zhang, Tao.,Sun, Lina.,Ru, Xiaoshang.,...&Yang, Hongsheng.(2016).Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea).INVERTEBRATE REPRODUCTION & DEVELOPMENT,60(2),131-136.
MLA Liu, Shilin,et al."Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)".INVERTEBRATE REPRODUCTION & DEVELOPMENT 60.2(2016):131-136.
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