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Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)
Liu, Shilin1; Zhou, Yi1; Zhang, Tao1; Sun, Lina1; Ru, Xiaoshang1; Li, Junhua2; Yang, Hongsheng1
2016-06-01
Source PublicationINVERTEBRATE REPRODUCTION & DEVELOPMENT
Volume60Issue:2Pages:131-136
SubtypeArticle
AbstractTechniques for in vitro oocyte maturation are important for studying maturation regulatory mechanisms, cell differentiation, and embryonic development. In this study, after Apostichopus japonicus gonads were dissected, oocytes, together with gonadal fluid extracted from gonads, were dried in shade. The effects of three temperatures (17, 21, and 25 degrees C) on oocyte maturation and following development were studied. The results showed that germinal vesicle breakdown (GVBD) of most oocytes occurred after desiccation, and those oocytes with GVBD could be fertilized and develop normally. There were no significant differences in the percentages of GVBD and individuals in blastula and gastrula stages between 17 and 21 degrees C (p > .05). The percentages of GVBD reached a maximum (17 degrees C: 98.6%, 21 degrees C: 97.1%) after 2 h of desiccation. The percentages of blastulae and gastrulae reached the maximum of more than 80% after 2.5-3.5 h of desiccation. At 25 degrees C, the percentages of GVBD, blastulae, and gastrulae reached a maximum of 76.7, 61.0, and 59.9% after 3.5 h of desiccation, respectively, and were significantly lower than that at 17 and 21 degrees C (p < .01). This study not only provided an effective and simple technique for in vitro induction of A. japonicus oocyte maturation, but also furthered understanding of gamete biology of this species.
KeywordApostichopus Japonicus Oocyte Gvbd Drying Development
DOI10.1080/07924259.2016.1174154
Indexed BySCI
Language英语
WOS IDWOS:000377424500007
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/126241
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Shilin,Zhou, Yi,Zhang, Tao,et al. Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT,2016,60(2):131-136.
APA Liu, Shilin.,Zhou, Yi.,Zhang, Tao.,Sun, Lina.,Ru, Xiaoshang.,...&Yang, Hongsheng.(2016).Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea).INVERTEBRATE REPRODUCTION & DEVELOPMENT,60(2),131-136.
MLA Liu, Shilin,et al."Effect of desiccation in shade on in vitro oocyte maturation and development of the sea cucumber Apostichopus japonicus (Echinodermata: Holothuroidea)".INVERTEBRATE REPRODUCTION & DEVELOPMENT 60.2(2016):131-136.
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