IOCAS-IR  > 实验海洋生物学重点实验室
Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus
Wang Xueying1,2; Shi Xuehui3; Liu Yifan1; Yu Daode5; Guan Shuguang5; Liu Qinghua1,4; Li Jun1,4
2016-07-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume34Issue:4Pages:763-771
SubtypeArticle
AbstractThe present study evaluated the effects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gr), and lipid peroxidation (measured via malondialdehyde (MDA) content) were determined after the milt was stored at 4 degrees C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol (PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation (only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could significantly improve the post-thaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had significant effects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.
KeywordPacific Cod Sperm Seminal Plasma Cryopreservation Sperm Physiology
DOI10.1007/s00343-016-5088-z
Indexed BySCI
Language英语
WOS IDWOS:000377207800013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/126231
Collection实验海洋生物学重点实验室
海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China
4.Chinese Acad Sci, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
5.Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang Xueying,Shi Xuehui,Liu Yifan,et al. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(4):763-771.
APA Wang Xueying.,Shi Xuehui.,Liu Yifan.,Yu Daode.,Guan Shuguang.,...&Li Jun.(2016).Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(4),763-771.
MLA Wang Xueying,et al."Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.4(2016):763-771.
Files in This Item:
File Name/Size DocType Version Access License
Effects of chilled s(1542KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang Xueying]'s Articles
[Shi Xuehui]'s Articles
[Liu Yifan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang Xueying]'s Articles
[Shi Xuehui]'s Articles
[Liu Yifan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang Xueying]'s Articles
[Shi Xuehui]'s Articles
[Liu Yifan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.