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Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus
Wang Xueying1,2; Shi Xuehui3; Liu Yifan1; Yu Daode5; Guan Shuguang5; Liu Qinghua1,4; Li Jun1,4
2016-07-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号34期号:4页码:763-771
文章类型Article
摘要The present study evaluated the effects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gr), and lipid peroxidation (measured via malondialdehyde (MDA) content) were determined after the milt was stored at 4 degrees C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol (PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation (only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could significantly improve the post-thaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had significant effects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.
关键词Pacific Cod Sperm Seminal Plasma Cryopreservation Sperm Physiology
DOI10.1007/s00343-016-5088-z
收录类别SCI
语种英语
WOS记录号WOS:000377207800013
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/126231
专题实验海洋生物学重点实验室
海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China
4.Chinese Acad Sci, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
5.Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China
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Wang Xueying,Shi Xuehui,Liu Yifan,et al. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(4):763-771.
APA Wang Xueying.,Shi Xuehui.,Liu Yifan.,Yu Daode.,Guan Shuguang.,...&Li Jun.(2016).Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(4),763-771.
MLA Wang Xueying,et al."Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.4(2016):763-771.
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