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Expression of hematopoietic transcription factors Runt, CBF beta and GATA during ontogenesis of scallop Chlamys farreri
Yue, Feng1,3; Wang, Lingling2; Wang, Hao1; Song, Linsheng2
2016-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume61Pages:88-96
SubtypeArticle
AbstractTranscription factors Runx1, CBF beta and GATA1/2/3 play essential roles in regulating hematopoietic development during embryogenesis of vertebrate. In previous study, the orthologous genes of Runt, CBF beta and GATA1/2/3 have been identified from scallop Chlamys farreri and proved to have conserved function in regulating hemocyte production. Here, these three transcription factors were selected as hematopoietic markers to explore potential developmental events of hematopoiesis during ontogenesis of scallop. The transcripts of CfRunt, CfCBF beta and CfGATA were detected abundantly after 32-cell embryo, trochophore and morula stage, and reached to a peak level in 32-cell embryos and D-shaped veligers, pediveligers or gastrula respectively. Further whole-mount immunofluorescence assay showed that the immunoreactivity of CfRunt was firstly observed at 32-cell stage and then its distribution was specialized gradually to the mesoderm during gastrulation. By trochophore, the expression of CfRunt, CfCBF beta and CfGATA proteins occurred coincidently in two specific symmetry cell mass located bilaterally on prototroch, and then disappeared rapidly in D-shaped or umbonal vliger, respectively. However, remarkable expressions of the three transcription factors were observed consistently in a new sinus structure appeared at the dorsal anterior side of D-shaped and umbonal veliger. After bacterial challenge, the mRNA expression levels of the three transcription factors were up-regulated or down-regulated significantly in trochophore, D-shaped veliger and pediveliger, indicating the available hematopoietic regulation in scallop larvae. The results revealed that scallop might experience two waves of hematopoiesis during early development, which occurred in the bilateral symmetry cell mass of trochophore and the sinus structure of veliger. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordChlamys Farreri Ontogenesis Transcription Factor Hematopoietic Development Bacterial Challenge
DOI10.1016/j.dci.2016.03.021
Indexed BySCI
Language英语
WOS IDWOS:000376804300010
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/126220
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yue, Feng,Wang, Lingling,Wang, Hao,et al. Expression of hematopoietic transcription factors Runt, CBF beta and GATA during ontogenesis of scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,61:88-96.
APA Yue, Feng,Wang, Lingling,Wang, Hao,&Song, Linsheng.(2016).Expression of hematopoietic transcription factors Runt, CBF beta and GATA during ontogenesis of scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,61,88-96.
MLA Yue, Feng,et al."Expression of hematopoietic transcription factors Runt, CBF beta and GATA during ontogenesis of scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 61(2016):88-96.
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